English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional importance of conserved domains in the flowering- time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants

Robson, F., Costa, M. M. R., Hepworth, S. R., Vizir, I., Pineiro, M., Reeves, P. H., et al. (2001). Functional importance of conserved domains in the flowering- time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants. Plant Journal, 28(6), 619-631.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3E42-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3E43-4
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Robson, F., Author
Costa, M. M. R., Author
Hepworth, S. R., Author
Vizir, I., Author
Pineiro, M., Author
Reeves, P. H.1, Author              
Putterill, J., Author
Coupland, G.1, Author              
Affiliations:
1Dept. of Plant Developmental Biology (George Coupland), MPI for Plant Breeding Research, Max Planck Society, ou_1113571              

Content

show
hide
Free keywords: Arabidopsis; flowering; photoperiod; B-box zinc finger
 Abstract: CONSTANS promotes flowering of Arabidopsis in response to long- day conditions. We show that CONSTANS is a member of an Arabidopsis gene family that comprises 16 other members. The CO-Like proteins encoded by these genes contain two segments of homology: a zinc finger containing region near their amino terminus and a CCT (CO, CO-Like, TOC1) domain near their carboxy terminus. Analysis of seven classical co mutant alleles demonstrated that the mutations all occur within either the: zinc finger region or the CCT domain, confirming that the two regions of homology are important for CO function. The zinc fingers are most similar to those of B-boxes, which act as protein-protein interaction domains in several transcription factors described in animals. Segments of CO protein containing the CCT domain localize GFP to the nucleus, but one mutation that affects the CCT domain delays flowering without affecting the nuclear localization function, suggesting that this domain has additional functions. All eight co alleles, including one recovered by pollen irradiation in which DNA encoding both B- boxes is deleted, are shown to be semidominant. This dominance appears to be largely due to a reduction in CO dosage in the heterozygous plants. However, some alleles may, also actively delay flowering, because overexpression from the CaMV 35S promoter of the co-3 allele, that has a mutation in the second B-box, delayed flowering of wild-type plants. The significance of these observations for the role of CO in the control of flowering time is discussed.

Details

show
hide
Language(s): eng - English
 Dates: 2001-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28828
ISI: 000173421000002
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Journal
  Alternative Title : Plant J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 28 (6) Sequence Number: - Start / End Page: 619 - 631 Identifier: ISSN: 0960-7412