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  Quantitative measurement of changes in neural activity in vivo utilizing dynamic manganese-enhanced MRI (dMEMRI)

Leuze, C., Kimura, Y., Kershaw, J., Shibata, S., Saga, T., Chuang, K.-H., et al. (2012). Quantitative measurement of changes in neural activity in vivo utilizing dynamic manganese-enhanced MRI (dMEMRI). NeuroImage, 60(1), 392-399. doi:10.1016/j.neuroimage.2011.12.030.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-624B-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CC6F-C
Genre: Journal Article

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 Creators:
Leuze, Christoph1, 2, 3, Author              
Kimura, Yuichi1, Author
Kershaw, Jeff1, Author
Shibata, Sayaka1, Author
Saga, Tsuneo1, Author
Chuang, Kai-Hsiang4, Author
Shimoyama, Ichiro3, Author
Aoki, Ichio1, Author
Affiliations:
1Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan, ou_persistent22              
2Department Neurophysics, MPI for Human Cognitive and Brain Sciences, Max Planck Society, Leipzig, DE, ou_634550              
3Research Center for Frontier Medical Engineering, Chiba University, Japan, ou_persistent22              
4Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Singapore, ou_persistent22              

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Free keywords: MEMRI; Manganese-enhanced MRI; Pituitary; Logan plot; Patlak plot; Kinetic analysis
 Abstract: The ability of manganese ions (Mn2+) to enter cells through calcium ion (Ca2+) channels has been used for depolarization dependent brain functional imaging with manganese-enhanced MRI (MEMRI). The purpose of this study was to quantify changes to Mn2+ uptake in rat brain using a dynamic manganese-enhanced MRI (dMEMRI) scanning protocol with the Patlak and Logan graphical analysis methods. The graphical analysis was based on a three-compartment model describing the tissue and plasma concentration of Mn. Mn2+ uptake was characterized by the total distribution volume of manganese (Mn) inside tissue (VT) and the unidirectional influx constant of Mn2+ from plasma to tissue (Ki). The measurements were performed on the anterior (APit) and posterior (PPit) parts of the pituitary gland, a region with an incomplete blood brain barrier. Modulation of Ca2+ channel activity was performed by administration of the stimulant glutamate and the inhibitor verapamil. It was found that the APit and PPit showed different Mn2+ uptake characteristics. While the influx of Mn2+ into the PPit was reversible, Mn2+ was found to be irreversibly trapped in the APit during the course of the experiment. In the PPit, an increase of Mn2+ uptake led to an increase in VT (from 2.8 ± 0.3 ml/cm3 to 4.6 ± 1.2 ml/cm3) while a decrease of Mn2+ uptake corresponded to a decrease in VT (from 2.8 ± 0.3 ml/cm3 to 1.4 ± 0.3 ml/cm3). In the APit, an increase of Mn2+ uptake led to an increase in Ki (from 0.034 ± 0.009 min− 1 to 0.049 ± 0.012 min− 1) while a decrease of Mn2+ uptake corresponded to a decrease in Ki (from 0.034 ± 0.009 min− 1 to 0.019 ± 0.003 min− 1). This work demonstrates that graphical analysis applied to dMEMRI data can quantitatively measure changes to Mn2+ uptake following modulation of neural activity.

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Language(s): eng - English
 Dates: 2011-12-152011-12-292012-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: PMID: 22227885
DOI: 10.1016/j.neuroimage.2011.12.030
Other: Epub 2011
 Degree: -

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Title: NeuroImage
Source Genre: Journal
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Publ. Info: Orlando, FL : Academic Press
Pages: - Volume / Issue: 60 (1) Sequence Number: - Start / End Page: 392 - 399 Identifier: ISSN: 1053-8119
CoNE: https://pure.mpg.de/cone/journals/resource/954922650166