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  High throughput object-based image analysis of β-amyloid plaques in human and transgenic mouse brain

Samaroo, H. D., Opsahl, A. C., Schreiber, J., O’Neill, S. M., Marconi, M., Qian, J., et al. (2012). High throughput object-based image analysis of β-amyloid plaques in human and transgenic mouse brain. Journal of Neuroscience Methods, 204(1), 179-188. doi:10.1016/j.jneumeth.2011.10.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-6335-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CE1B-8
Genre: Journal Article

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 Creators:
Samaroo, Harry D.1, Author
Opsahl, Alan C.2, Author
Schreiber, Jan3, Author              
O’Neill, Sharon M.1, Author
Marconi, Michael1, Author
Qian, Jessie2, Author
Carvajal-Gonzalez, Santos1, Author
Stephenson, Diane T.1, Author
Affiliations:
1Neuroscience Biology, Pfizer Global Research & Development, Groton, CT, USA, ou_persistent22              
2Investigative Pathology, Pfizer Global Research & Development, Groton, CT, USA, ou_persistent22              
3Definiens AG, Munich, Germany, ou_persistent22              

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Free keywords: High-throughput; PS1APP; Object-based image analysis; Alzheimer's disease
 Abstract: Advances in imaging technology have enabled automated approaches for quantitative image analysis. In this study, a high content object based image analysis method was developed for quantification of β-amyloid (Aβ) plaques in postmortem brains of Alzheimer's disease (AD) subjects and in transgenic mice over overexpressing Aβ. Digital images acquired from immunohistochemically stained sections of the superior frontal gyrus were analyzed for Aβ plaque burden using a Definiens object-based segmentation approach. Blinded evaluation of Aβ stained sections from AD and aged matched human subjects accurately identified AD cases with one exception. Brains from transgenic mice overexpressing Aβ (PS1APP mice) were also evaluated by our Definiens object based image analysis approach. We observed an age-dependent increase in the amount of Aβ plaque load that we quantified in both the hippocampus and cortex. From the contralateral hemisphere, we measured the amount of Aβ in brain homogenates biochemically and observed a significant correlation between our biochemical measurements and those that we measured by our object based Definiens system in the hippocampus. Assessment of Aβ plaque load in PS1APP mice using a manual segmentation technique (Image-Pro Plus) confirmed the results of our object-based image analysis approach. Image acquisition and analysis of 32 stained human slides and 100 mouse slides were executed in 8 h and 22 h, respectively supporting the relatively high throughput features of the Definiens platform. The data show that digital imaging combined with object based image analysis is a reliable and efficient approach to quantifying Aβ plaques in human and mouse brain.

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Language(s): eng - English
 Dates: 2011-03-292011-10-072011-10-122012-02-15
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.jneumeth.2011.10.003
PMID: 22019329
Other: Epub 2011
 Degree: -

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Title: Journal of Neuroscience Methods
  Other : J. Neurosci. Meth.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 204 (1) Sequence Number: - Start / End Page: 179 - 188 Identifier: ISSN: 0165-0270
CoNE: https://pure.mpg.de/cone/journals/resource/954925480594