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  Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury

Ertürk, A., Mauch, C. P., Hellal, F., Förstner, F., Keck, T., Becker, K., et al. (2012). Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nature Medicine, 18(1), 166-171. doi:10.1038/nm.2600.

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Ertürk, Ali1, Autor           
Mauch, Christoph P., Autor
Hellal, Farida1, Autor           
Förstner, Friedrich2, Autor           
Keck, Tara3, Autor           
Becker, Klaus, Autor
Jaehrling, Nina, Autor
Steffens, Heinz, Autor
Richter, Melanie, Autor
Hübener, Mark3, Autor           
Kramer, Edgar, Autor
Kirchhoff, Frank, Autor
Dodt, Hans Ulrich, Autor
Bradke, Frank1, Autor           
Affiliations:
1Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society, ou_1113553              
2Department: Systems and Computational Neurobiology / Borst, MPI of Neurobiology, Max Planck Society, ou_1113548              
3Department: Cellular and Systems Neurobiology / Bonhoeffer, MPI of Neurobiology, Max Planck Society, ou_1113545              

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Schlagwörter: NOGO-A; FUNCTIONAL RECOVERY; TRANSGENIC MICE; ANTIBODY IN-1; ASTROCYTES; PROTEIN; LESION; BRAIN; DEGENERATION; FIBERSBiochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental;
 Zusammenfassung: Studying regeneration in the central nervous system (CNS) is hampered
by current histological and imaging techniques because they provide
only partial information about axonal and glial reactions. Here we
developed a tetrahydrofuranbased clearing procedure that renders fixed
and unsectioned adult CNS tissue transparent and fully penetrable for
optical imaging. In large spinal cord segments, we imaged fluorescently
labeled cells by `ultramicroscopy' and two-photon microscopy without
the need for histological sectioning. We found that more than a year
after injury growth-competent axons regenerated abundantly through the
injury site. A few growth-incompetent axons could also regenerate when
they bypassed the lesion. Moreover, we accurately determined
quantitative changes of glial cells after spinal cord injury. Thus,
clearing CNS tissue enables an unambiguous evaluation of axon
regeneration and glial reactions. Our clearing procedure also renders
other organs transparent, which makes this approach useful for a large
number of preclinical paradigms.

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Sprache(n): eng - English
 Datum: 2012-01-01
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000299018600044
DOI: 10.1038/nm.2600
 Art des Abschluß: -

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Titel: Nature Medicine
  Andere : Nat. Med.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: New York, NY : Nature Pub. Co.
Seiten: - Band / Heft: 18 (1) Artikelnummer: - Start- / Endseite: 166 - 171 Identifikator: ISSN: 1078-8956
CoNE: https://pure.mpg.de/cone/journals/resource/954925606824