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Free keywords:
beta-glucan-binding protein
glucan affinity purification
peptide-specific antiserum
soybean
high-affinity binding
glucoside phytoalexin elicitor
membranes
fractions
Abstract:
A low abundance beta-glucan elicitor-binding protein from soybean was isolated by a rapid, simple and one-step purification method yielding about 9000-fold enrichment, The affinity-based purification technique was more efficient than a procedure that uses conventional methods and preserved the binding activity to a much larger extent, The final preparation consisted of one major protein with an apparent molecular mass of about 75 kDa, Electrophoretic analyses of the purified and photoaffinity-labeled binding protein showed that the native protein was an oligomer with apparent molecular mass of about 240 kDa, A polyclonal anti-peptide antiserum was raised against a synthetic 15-mer internal oligopeptide sequence derived from the 75-kDa protein, The antiserum recognized the purified binding protein in immunoblotting experiments and precipitated the affinity-labeled protein from a crude extract of the membrane fraction.
