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  Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.

Schmitzova, J., Rasche, N., Dybkov, O., Kramer, K., Fabrizio, P., Urlaub, H., et al. (2012). Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein. EMBO Journal, 31(9), 2222-2234. doi:10.1038/emboj.2012.58.

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Schmitzova, J.1, Autor           
Rasche, N.1, Autor           
Dybkov, O.1, Autor           
Kramer, K.2, Autor           
Fabrizio, P.1, Autor           
Urlaub, H.2, Autor           
Lührmann, R.1, Autor           
Pena, V.3, Autor           
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
3Research Group of Macromolecular Crystallography, MPI for Biophysical Chemistry, Max Planck Society, ou_2035293              

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 Zusammenfassung: The yeast splicing factor Cwc2 contacts several catalytically important RNA elements in the active spliceosome, suggesting that Cwc2 is involved in determining their spatial arrangement at the spliceosome's catalytic centre. We have determined the crystal structure of the Cwc2 functional core, revealing how a previously uncharacterized Torus domain, an RNA recognition motif (RRM) and a zinc finger (ZnF) are tightly integrated in a compact folding unit. The ZnF plays a pivotal role in the architecture of the whole assembly. UV-induced crosslinking of Cwc2–U6 snRNA allowed the identification by mass spectrometry of six RNA-contacting sites: four in or close to the RRM domain, one in the ZnF and one on a protruding element connecting the Torus and RRM domains. The three distinct regions contacting RNA are connected by a contiguous and conserved positively charged surface, suggesting an expanded interface for RNA accommodation. Cwc2 mutations confirmed that the connector element plays a crucial role in splicing. We conclude that Cwc2 acts as a multipartite RNA-binding platform to bring RNA elements of the spliceosome's catalytic centre into an active conformation.

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Sprache(n): eng - English
 Datum: 2012-03-092012
 Publikationsstatus: Erschienen
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 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/emboj.2012.58
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Titel: EMBO Journal
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 31 (9) Artikelnummer: - Start- / Endseite: 2222 - 2234 Identifikator: ISSN: 0261-4189