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  Assembly and characterization of protein multi-layer systems

Christensen, D. (2004). Assembly and characterization of protein multi-layer systems. Master Thesis, Johannes Gutenberg-Universität, Mainz.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-6080-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-6081-F
Genre: Thesis

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 Creators:
Christensen, Danica1, Author              
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1MPI for Polymer Research, Max Planck Society, ou_1309545              

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 Abstract: Abstract: Biocompatible multi-layer architectures are of great interest for the construction of biosensor applications. We have established a system that is a multi-layer protein architecture with alternating layers of streptavidin (SA) and biotinylated immunoglobulin G (B-IgG). The layer building properties of this protein multi-layer architecture has been studied in situ using the surface plasmon resonance spectroscopy (SPR). Studies were also done using neutron reflectivity to investigate the surface architecture of the multi-layers. The multi-layer architecture has been shown to assemble with a linear growth of the multi-layer thickness with respect to the number of layers deposited. Experiments conducted using SPR have also shown that the multi-layers are stable against free biotin attack and the assembly of the multi-layers is very reproducible. The effects of using SA versus avidin to build the multi-layer system were studied using SPR. Two immobilization techniques for the attachment of the first SA layer to the metal surface were compared with SPR; the first using covalent bonding between the SA and a thiol derivative self-assembled to the surface, the second technique used the binding affinity of SA and biotin to bind the SA directly to a biotinylated thiol on the gold surface. The fluorescence intensity of Alexa fluoro labeled streptavidin (AFSA) was investigated using Surface Plasmon Fluorescence Spectroscopy (SPFS). The effect of adding additional non-labeled protein layers to a layer of AFSA that was placed close to the gold surface, about 3 nm, and a second layer a bit further away from the metal surface, at 15 nm, on the fluorescence intensity was also investigated using SPFS.

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Language(s): eng - English
 Dates: 2004
 Publication Status: Accepted / In Press
 Pages: -
 Publishing info: Mainz : Johannes Gutenberg-Universität
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 205896
Other: D 6/04
 Degree: Master

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