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  Lateral diffusion in substrate-supported lipid monolayers as a function of ambient relative humidity

Baumgart, T., & Offenhäusser, A. (2002). Lateral diffusion in substrate-supported lipid monolayers as a function of ambient relative humidity. Biophysical Journal, 83(3), 1489-1500. doi:10.1016/S0006-3495(02)73919-2.

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 Creators:
Baumgart, T.1, Author              
Offenhäusser, Andreas1, Author              
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

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 Abstract: We analyzed the influence of water activity on the lateral self-diffusion of supported phospholipid monolayers. Lipid monolayer membranes were supported by polysaccharide cushions (chitosan and agarose), or glass. A simple diffusion model was derived, based on activated diffusion with an activation energy, E-a, which depends on the hydration state of the lipid headgroup. A crucial assumption of the derived model is that E- a can be calculated assuming an exponential decay of the humidity-dependent disjoining pressure in the monolayer/substrate interface with respect to the equilibrium separation distance. A plot of In(D) against In(p(o)/p), where D is the measured diffusion coefficient and p(o) and p are the partial water pressures at saturation and at a particular relative humidity, respectively, was observed to be linear in all cases (i.e., for differing lipids, lateral monolayer pressures, temperatures, and substrates), in accordance with the above-mentioned diffusion model. No indications for humidity-induced first-order phase transitions in the supported phospholipid monolayers were found. Many biological processes such as vesicle fusion and recognition processes involve dehydration/hydration cycles, and it can be expected that the water activity significantly affects the kinetics of these processes in a manner similar to that examined in the present work.

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Language(s): eng - English
 Dates: 2002-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: No review
 Identifiers: eDoc: 28304
ISI: 000177774500024
DOI: 10.1016/S0006-3495(02)73919-2
 Degree: -

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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 83 (3) Sequence Number: - Start / End Page: 1489 - 1500 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117