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  Sensitivity studies for specific binding reactions using the biotin/streptavidin system by evanescent optical methods

Busse, S., Scheumann, V., Menges, B., & Mittler, S. (2002). Sensitivity studies for specific binding reactions using the biotin/streptavidin system by evanescent optical methods. Biosensors & Bioelectronics, 17(8), 704-710.

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Busse, S., Author
Scheumann, V.1, Author              
Menges, B.1, Author              
Mittler, Silvia1, Author              
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

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Free keywords: evanescent wave optics; surface plasmon spectroscopy; waveguide mode spectroscopy; Mach-Zehnder interferometer; AFM; biotinstreptavidin binding; antigen-antibody binding; refractive index; thickness of swollen streptavidin
 Abstract: The combination of various evanescent optical methods such as surface plasmon spectroscopy, waveguide mode spectroscopy and an integrated optical Mach - Zelander-interferometer are used to characterize biotinylated self-assembled monolayers as well as the binding of streptavidin to these labels. The aim of designing a highly specific and sensitive, re-usable affinity sensor for antigens on the basis of an integrated optical Mach- Zehnder interferometer is based on a proper understanding of the characteristics of the entire binding matrix architecture. Therefore, a variety of biotin-derivatives immobilized in a monolayer are investigated with respect to their affinity to streptavidin and the possibility to remove the steptavidin layer specifically. The density of the streptavidin layer as well as the optical constants of the involved molecules are measured. Finally the integrated optical Mach-Zehnder interferometer is tested with respect to the sensitivity to an antigen-antibody binding reaction. An attempt to further increase the sensitivity by simultaneous detection of a fluorescence signal failed due to bleaching effects. (C) 2002 Elsevier Science B.V. All rights reserved.

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Language(s): eng - English
 Dates: 2002-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: eDoc: 28288
ISI: 000176900000009
Other: P-02-71
 Degree: -

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Title: Biosensors & Bioelectronics
  Alternative Title : Biosens. Bioelectron.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 17 (8) Sequence Number: - Start / End Page: 704 - 710 Identifier: ISSN: 0956-5663