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  Stam2 expression pattern during embryo development.

Curlin, M., Kapuralin, K., Muro, A. F., Baralle, F. E., Chowdhury, K., & Gajovic, S. (2012). Stam2 expression pattern during embryo development. Gene Expression Patterns, 12(1-2), 68-76. doi:10.1016/j.gep.2011.11.005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-842F-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CAF6-0
Genre: Journal Article

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1448916.pdf (Publisher version), 2MB
 
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 Creators:
Curlin, M., Author
Kapuralin, K., Author
Muro, A. F., Author
Baralle, F. E., Author
Chowdhury, K.1, Author              
Gajovic, S., Author
Affiliations:
1Facility of Microarray Analyses, MPI for biophysical chemistry, Max Planck Society, ou_578589              

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Free keywords: STAM2; Gene trap; Mouse; Embryo; Expression; X-gal staining; Development
 Abstract: STAM2 is a tyrosine-phosphorylated protein suggested to be involved in cargo selection during endocytic pathway, regulation of exocytosis and intracellular signaling. Gene trap method was used to create via insertional mutagenesis a mutant mouse line with integration of promoterless bgeo (lacZ–neomycin phosphotransferase fusion) gene in the second intron of Stam2 gene, enabling analysis of its in vivo expression and function. The inserted b-galactosidase (lacZ) reporter gene was used to reveal Stam2 expression during development. Stam2 in situ RNA hybridization and immunostaining confirmed the observed b-galactosidase activity reflecting high Stam2 expression. The homozygous mutant mice showed no overt phenotypic alterations. Stam2 expression was detected after E9.5 in the gut, notochord, neural tube and heart. In the nervous system it was located in the floor, roof and basal plates of the developing neural tube, and in the developing cortex, hippocampus and olfactory bulbs. Toward the end of gestation, Stam2 expression appeared in the testis and ovary, lungs, nasal cavity epithelium, kidneys, urogenital sinus, intestine, pancreas, pituitary and adrenal glands, muscles, brown adipose tissue, skin and epithelium of the tongue and oral cavity.

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Language(s): eng - English
 Dates: 2011-11-282012-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.gep.2011.11.005
 Degree: -

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Title: Gene Expression Patterns
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 12 (1-2) Sequence Number: - Start / End Page: 68 - 76 Identifier: ISSN: 1567-133X
CoNE: /journals/resource/954925385137