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  Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter.

Chua, J. J. E., Butkevich, E., Worseck, J. M., Kittelmann, M., Gronborg, M., Behrmann, E., et al. (2012). Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter. Proceedings of the National Academy of Sciences of the United States of America, 109(15), 5862-5867. doi:10.1073/pnas.1113819109.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-84CB-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8137-C
Genre: Journal Article

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Chua, J. J. E.1, Author              
Butkevich, E.2, Author              
Worseck, J. M., Author
Kittelmann, M., Author
Gronborg, M.2, Author              
Behrmann, E.2, Author              
Stelzl, U., Author
Pavlos, N. J., Author
Lalowski, M. M., Author
Eimer, S., Author
Wanker, E. E., Author
Klopfenstein, D. R., Author
Jahn, R.2, Author              
Affiliations:
1Research Group of Protein Trafficking in Synaptic Development and Function, MPI for Biophysical Chemistry, Max Planck Society, ou_1933287              
2Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

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 Abstract: Presynaptic nerve terminals are formed from preassembled vesicles that are delivered to the prospective synapse by kinesin-mediated axonal transport. However, precis ely how the various cargoes are linked to the motor proteins remains unclear. Here, we report a transport complex linking syntax in 1a (Stx) and Munc18, two pro- teins functioning in synaptic vesicle exocytosis at the presynaptic plasma membrane, to the motor protein Kinesin-1 via the kinesin adaptor FEZ1. Mutation of the FEZ1 ortholog UNC-76 in Caenorhabdi- tis elegans causes defects in the axonal transport of Stx. We also show that binding of FEZ1 to Kinesin-1 and Munc18 is regulated by phos- phorylation, with a conserved site (s erine 58) being essential for bind- ing.Whenexpressedin C. elegans ,wild-typebutnotphosphorylation- de fi cient FEZ1 (S58A) restored axonal transport of Stx. We conclude that FEZ1 operates as a kinesin adaptor for the transport of Stx, with cargo loading and unloading being regulated by protein kinases.

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Language(s): eng - English
 Dates: 2012-03-262012-04-10
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1113819109
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Title: Proceedings of the National Academy of Sciences of the United States of America
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Source Genre: Journal
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Publ. Info: National Academy of Sciences
Pages: - Volume / Issue: 109 (15) Sequence Number: - Start / End Page: 5862 - 5867 Identifier: ISSN: 0027-8424
CoNE: /journals/resource/954925427230