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  Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

Sanchis, J., Fernández, L., Carballeira, J. D., Drône, J., Gumulya, Y., Höbenreich, H., et al. (2008). Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates. Applied Microbiology and Biotechnology, 81, 387-397. doi:10.1007/s00253-008-1678-9.

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 Creators:
Sanchis, J.1, Author
Fernández, L.2, Author              
Carballeira, J. D.1, Author
Drône, J.2, Author              
Gumulya, Y.2, Author              
Höbenreich, H.2, Author              
Kahakeaw, D.2, Author              
Kille, S.2, Author              
Lohmer, R.2, Author              
Peyralans, J. J.-P.2, Author              
Podtetenieff, J.2, Author              
Prasad, S.2, Author              
Soni, P.2, Author              
Taglieber, A.2, Author              
Wu, S.2, Author              
Zilly, F. E.2, Author              
Reetz, M. T.2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              

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Free keywords: Directed evolution; Saturation mutagenesis; PCR; Megaprimer; Antiprimer; Difficult-to-amplify templates
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Language(s): eng - English
 Dates: 2008
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 408420
DOI: 10.1007/s00253-008-1678-9
ISI: 000260635400020
 Degree: -

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Title: Applied Microbiology and Biotechnology
  Alternative Title : Appl. Microbiol. Biotechnol.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 81 Sequence Number: - Start / End Page: 387 - 397 Identifier: ISSN: 0175-7598