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  Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

Sanchis, J., Fernández, L., Carballeira, J. D., Drône, J., Gumulya, Y., Höbenreich, H., et al. (2008). Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates. Applied Microbiology and Biotechnology, 81, 387-397. doi:10.1007/s00253-008-1678-9.

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 Creators:
Sanchis, J.1, Author
Fernández, L.2, Author           
Carballeira, J. D.1, Author
Drône, J.2, Author           
Gumulya, Y.2, Author           
Höbenreich, H.2, Author           
Kahakeaw, D.2, Author           
Kille, S.2, Author           
Lohmer, R.2, Author           
Peyralans, J. J.-P.2, Author           
Podtetenieff, J.2, Author           
Prasad, S.2, Author           
Soni, P.2, Author           
Taglieber, A.2, Author           
Wu, S.2, Author           
Zilly, F. E.2, Author           
Reetz, M. T.2, Author           
Affiliations:
1Max Planck Society, ou_persistent13              
2Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              

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Free keywords: Directed evolution; Saturation mutagenesis; PCR; Megaprimer; Antiprimer; Difficult-to-amplify templates
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Language(s): eng - English
 Dates: 2008
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 408420
DOI: 10.1007/s00253-008-1678-9
ISI: 000260635400020
 Degree: -

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Title: Applied Microbiology and Biotechnology
  Alternative Title : Appl. Microbiol. Biotechnol.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 81 Sequence Number: - Start / End Page: 387 - 397 Identifier: ISSN: 0175-7598