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  Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection

Belder, D., Deege, A., Maass, M., & Ludwig, M. (2002). Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection. Electrophoresis, 23(14), 2355-2361. doi:10.1002/1522-2683(200207)23:14<2355:AID-ELPS2355>3.0.CO;2-Q.

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 Creators:
Belder, Detlev1, Author              
Deege, Alfred1, Author              
Maass, Martin2, Author
Ludwig, Martin1, Author              
Affiliations:
1Research Group Belder, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445595              
2Olympus Optical Co. (Europa), Abt. Mikroskopie Deutschland, Hamburg, Germany, ou_persistent22              

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Free keywords: Chiral amines; Chiral separation; Fluorescence detection; Microchip electrophoresis; Variable wavelength
 Abstract: A modular instrument for high-speed microchip electrophoresis (MCE) equipped with a sensitive variable-wavelength fluorescence detection system was developed and evaluated. The experimental setup consists mainly of a lamp-based epifluorescence microscope for variable-wavelength fluorescence detection and imaging and a programmable four-channel bipolar high-voltage source capable of delivering up to +/- 10 W per channel. The optical unit was equipped with a high-sensitivity photomultiplier tube and an adjustable aperture. The system was applied to MCE separations of flurescein isothiocyanate (FITC)- labelled amines utilizing blue light (450-480 nm) for excitation as well as for the separation of rhodamines utilizing excitation light in the green spectral region (531- 560 nm). At optimized conditions baseline separation of four FITC-labelled amines could be obtained in less than 50 s at a detection limit of 460 ppt (1 nM) with a signal-to-noise ratio of 3:1. Three rhodamines could be baseline-separated in less than 6 s at a detection limit of 240 ppt (500 pm). The relative standard deviations of absolute migration times determined in repetitive MCE separations of FITC-labelled amines were below 2.5% (n= 25). By the application of cyclodextrin-modified electrolytes, chiral separation of FITC-labelled amines could be performed in seconds demonstrating the potential of microchip electrophoresis for chiral high-throughput screening.

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Language(s): eng - English
 Dates: 2002-07-14
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Electrophoresis
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Verlag Chemie,
Pages: - Volume / Issue: 23 (14) Sequence Number: - Start / End Page: 2355 - 2361 Identifier: ISSN: 0173-0835
CoNE: https://pure.mpg.de/cone/journals/resource/954925486765