Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection

Belder, D., Deege, A., Maass, M., & Ludwig, M. (2002). Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection. Electrophoresis, 23(14), 2355-2361. doi:10.1002/1522-2683(200207)23:14<2355:AID-ELPS2355>3.0.CO;2-Q.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Belder, Detlev1, Autor           
Deege, Alfred1, Autor           
Maass, Martin2, Autor
Ludwig, Martin1, Autor           
Affiliations:
1Research Group Belder, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445595              
2Olympus Optical Co. (Europa), Abt. Mikroskopie Deutschland, Hamburg, Germany, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Chiral amines; Chiral separation; Fluorescence detection; Microchip electrophoresis; Variable wavelength
 Zusammenfassung: A modular instrument for high-speed microchip electrophoresis (MCE) equipped with a sensitive variable-wavelength fluorescence detection system was developed and evaluated. The experimental setup consists mainly of a lamp-based epifluorescence microscope for variable-wavelength fluorescence detection and imaging and a programmable four-channel bipolar high-voltage source capable of delivering up to +/- 10 W per channel. The optical unit was equipped with a high-sensitivity photomultiplier tube and an adjustable aperture. The system was applied to MCE separations of flurescein isothiocyanate (FITC)- labelled amines utilizing blue light (450-480 nm) for excitation as well as for the separation of rhodamines utilizing excitation light in the green spectral region (531- 560 nm). At optimized conditions baseline separation of four FITC-labelled amines could be obtained in less than 50 s at a detection limit of 460 ppt (1 nM) with a signal-to-noise ratio of 3:1. Three rhodamines could be baseline-separated in less than 6 s at a detection limit of 240 ppt (500 pm). The relative standard deviations of absolute migration times determined in repetitive MCE separations of FITC-labelled amines were below 2.5% (n= 25). By the application of cyclodextrin-modified electrolytes, chiral separation of FITC-labelled amines could be performed in seconds demonstrating the potential of microchip electrophoresis for chiral high-throughput screening.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2002-07-14
 Publikationsstatus: Erschienen
 Seiten: 7
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Electrophoresis
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Weinheim, Germany : Verlag Chemie,
Seiten: - Band / Heft: 23 (14) Artikelnummer: - Start- / Endseite: 2355 - 2361 Identifikator: ISSN: 0173-0835
CoNE: https://pure.mpg.de/cone/journals/resource/954925486765