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  Real-time assembly landscape of bacterial 30S translation initiation complex.

Milon, P., Maracci, C., Filonava, L., Gualerzi, C. O., & Rodnina, M. V. (2012). Real-time assembly landscape of bacterial 30S translation initiation complex. Nature Structural and Molecular Biology, 19(6), 609-615. doi:10.1038/nsmb.2285.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-9BC6-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1DDD-8
Genre: Journal Article

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 Creators:
Milon, P.1, Author              
Maracci, C., Author
Filonava, L.1, Author              
Gualerzi, C. O., Author
Rodnina, M. V.1, Author              
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1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: Initiation factors guide the ribosome in the selection of mRNA and translational reading frame. We determined the kinetically favored assembly pathway of the 30S preinitiation complex (30S PIC), an early intermediate in 30S initiation complex formation in Escherichia coli. IF3 and IF2 are the first factors to arrive, forming an unstable 30S–IF2–IF3 complex. Subsequently, IF1 joins and locks the factors in a kinetically stable 30S PIC to which fMet-tRNAfMet is recruited. Binding of mRNA is independent of initiation factors and can take place at any time during 30S PIC assembly, depending on the cellular concentration of the mRNA and the structural determinants at the ribosome-binding site. The kinetic analysis shows both specific and cumulative effects of initiation factors as well as kinetic checkpoints of mRNA selection at the entry into translation.

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Language(s): eng - English
 Dates: 2012-05-062012
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.2285
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 19 (6) Sequence Number: - Start / End Page: 609 - 615 Identifier: -