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  Heterochromatin protein 1 forms distinct complexes to direct histone deacetylation and DNA methylation

Honda, S., Lewis, Z., Shimada, K., Fischle, W., Sack, R., & Selker, E. (2012). Heterochromatin protein 1 forms distinct complexes to direct histone deacetylation and DNA methylation. Nature Structural and Molecular Biology, 19(5), 471-477. doi:10.1038/nsmb.2274.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-9CBC-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1DE2-B
Genre: Journal Article

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Honda, S., Author
Lewis, Z.A., Author
Shimada, K., Author
Fischle, W.1, Author              
Sack, R., Author
Selker, E.U., Author
Affiliations:
1Research Group of Chromatin Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578604              

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 Abstract: DNA methylation, methylation of histone H3 at Lys9 (H3K9me3) and hypoacetylated histones are common molecular features of heterochromatin. Important details of their functions and inter-relationships remain unclear, however. In Neurospora crassa, H3K9me3 directs DNA methylation through a complex containing heterochromatin protein 1 (HP1) and the DNA methyltransferase DIM-2. We identified a distinct HP1 complex, HP1, CDP-2, HDA-1 and CHAP (HCHC), and found that it is responsible for silencing independently of DNA methylation. HCHC defects cause hyperacetylation of centromeric histones, greater accessibility of DIM-2 and hypermethylation of centromeric DNA. Loss of HCHC also causes mislocalization of the DIM-5 H3K9 methyltransferase at a subset of interstitial methylated regions, leading to selective DNA hypomethylation. We demonstrate that HP1 forms distinct DNA methylation and histone deacetylation complexes that work in parallel to assemble silent chromatin in N. crassa.

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Language(s): eng - English
 Dates: 2012-04-152012
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.2274
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Publ. Info: New York, NY : Nature Publ. Group
Pages: - Volume / Issue: 19 (5) Sequence Number: - Start / End Page: 471 - 477 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763