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  Hyperpolarized binding pocket nuclear Overhauser effect for determination of competitive ligand binding

Lee, Y., Zeng, H., Mazur, A., Wegstroh, M., Carlomagno, T., Reese, M., et al. (2012). Hyperpolarized binding pocket nuclear Overhauser effect for determination of competitive ligand binding. Angewandte Chemie International Edition, 51(21), 5179-5182. doi:10.1002/anie.201201003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-9E66-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C741-D
Genre: Journal Article

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1478442.pdf (Publisher version), 425KB
 
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 Creators:
Lee , Y., Author
Zeng, H., Author
Mazur, A.1, Author              
Wegstroh, M.1, Author              
Carlomagno, T., Author
Reese, M.1, Author              
Lee, D.1, Author              
Becker, S.1, Author              
Griesinger, C.1, Author              
Hilty, C., Author
Affiliations:
1Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Hyperpolarization; ligands; NMR spectroscopy; proteins
 Abstract: Protein-mediated polarization transfer: Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin-polarization transfer through the binding site of the protein. If protons HL1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build-up of signal of the second ligand yields information on its binding epitope.

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Language(s): eng - English
 Dates: 2012-04-122012-05-21
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201201003
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 51 (21) Sequence Number: - Start / End Page: 5179 - 5182 Identifier: -