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  Distinct subsets of syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.

Dean, C., Liu, H., Staudt, T., Stahlberg, M. A., Vingill, S., Bückers, J., et al. (2012). Distinct subsets of syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity. The Journal of Neuroscience, 32(16), 5398-5413. doi:10.1523/JNEUROSCI.4515-11.2012.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-9F72-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C71D-0
Genre: Journal Article

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 Creators:
Dean, C., Author
Liu, H., Author
Staudt, T.1, Author              
Stahlberg, M. A., Author
Vingill, S., Author
Bückers, J.1, Author              
Kamin , D., Author
Engelhardt, J., Author
Jackson, M. B., Author
Hell, S. W.1, Author              
Chapmann, E. R., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, a phenomenon that underlies learningandmemory.However,whetherBDNFactsinadiffusemanneroristargetedtospecificneuronalsubcompartmentsorsynaptic sites to affect circuit function remains unknown. Here, using photoactivation of BDNF or syt-IV (a regulator of exocytosis present on BDNF-containing vesicles) in transfected rat hippocampal neurons, we discovered that distinct subsets of BDNF vesicles are targeted to axonsversusdendritesandarenotsharedbetweenthesecompartments.Moreover,syt-IV-andBDNF-harboringvesiclesarerecruitedto both presynaptic and postsynaptic sites in response to increased neuronal activity. Finally, using syt-IV knockout mouse neurons, we found that syt-IV is necessary for both presynaptic and postsynaptic scaling of synaptic strength in response to changes in network activity. These findings demonstrate that BDNF-containing vesicles can be targeted to specific sites in neurons and suggest that syt-IV- regulated BDNF secretion is subject to spatial control to regulate synaptic function in a site-specific manner.

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Language(s): eng - English
 Dates: 2012-04-18
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1523/JNEUROSCI.4515-11.2012
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Title: The Journal of Neuroscience
Source Genre: Journal
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Pages: - Volume / Issue: 32 (16) Sequence Number: - Start / End Page: 5398 - 5413 Identifier: -