English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Exceeding the limit of dynamics studies on biomolecules using high spin-lock field strengths with a cryogenically cooled probehead.

Ban, D., Gossert, A. D., Giller, K., Becker, S., Griesinger, C., & Lee, D. (2012). Exceeding the limit of dynamics studies on biomolecules using high spin-lock field strengths with a cryogenically cooled probehead. Journal of Magnetic Resonance, 221, 1-4. doi:10.1016/j.jmr.2012.05.005.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-9FF7-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-F08B-F
Genre: Journal Article

Files

show Files
hide Files
:
1478901.pdf (Publisher version), 397KB
Name:
1478901.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1478901_Supplement_1.doc (Supplementary material), 185KB
Name:
1478901_Supplement_1.doc
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/msword / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Ban, D., Author
Gossert, A. D., Author
Giller, K.1, Author              
Becker, S.1, Author              
Griesinger, C.1, Author              
Lee, D., Author
Affiliations:
1Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: Cryo-probe; Protein dynamics; Rotating-frame transverse relaxation; Relaxation dispersion; Ubiquitin
 Abstract: Internal motions in the microsecond timescale have been proposed to play an active part in a protein’s biological function. Nuclear magnetic resonance (NMR) relaxation dispersion is a robust method sensitive to this timescale with atomic resolution. However, due to technical limitations, the observation of motions faster than 40 l s for 15 N nuclei was not possible. We show that with a cryogenically cooled NMR probehead, a high spin-lock field strength can be generated that is able to detect motions as fast as 25 l s. We apply this high spin-lock field strength in an NMR experiment used for characterizing dynamical processes. An on-resonance rotating-frame transverse relaxation experiment was imple- mented that allows for the detection of a 25 l s process from a dispersion curve, and transverse relaxation rates were compared at low and high spin-lock field strengths showing that at high field strengths con- tributions from chemical exchange with lifetimes up to 25 l s can be removed. Due to the increase in sen- sitivity towards fast motion, relaxation dispersion for a residue that undergoes smaller chemical shift variations due to dynamics was identified. This technique reduces the previously inaccessible window between the correlation time and the relaxation dispersion window that covers four orders of magnitude by a factor of 2.

Details

show
hide
Language(s): eng - English
 Dates: 2012-05-142012-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jmr.2012.05.005
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Magnetic Resonance
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 221 Sequence Number: - Start / End Page: 1 - 4 Identifier: -