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  The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.

Karpiuk, O., Najafova, Z., Kramer, F., Hennion, M., Galonska, C., König, A., et al. (2012). The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells. Molecular Cell, 46(5), 705-713. doi:10.1016/j.molcel.2012.05.022.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-A4B2-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1D5C-C
Genre: Journal Article

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 Creators:
Karpiuk, O., Author
Najafova, Z., Author
Kramer, F., Author
Hennion, M., Author
Galonska, C., Author
König, A.1, Author              
Snaidero, N., Author
Vogel, T., Author
Shchebet, A., Author
Begus-Nahrmann, Y., Author
Kassem, M., Author
Simons, M., Author
Shcherbata, H. R.1, Author              
Beissbarth, T., Author
Johnsen, S. A., Author
Affiliations:
1Research Group of Gene Expression and Signaling, MPI for biophysical chemistry, Max Planck Society, ou_578608              

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 Abstract: Extensive changes in posttranslational histone modifications accompany the rewiring of the transcriptional program during stem cell differentiation. However, the mechanisms controlling the changes in specific chromatin modifications and their function during differentiation remain only poorly understood. We show that histone H2B monoubiquitination (H2Bub1) significantly increases during differentiation of human mesenchymal stem cells (hMSCs) and various lineage-committed precursor cells and in diverse organisms. Furthermore, the H2B ubiquitin ligase RNF40 is required for the induction of differentiation markers and transcriptional reprogramming of hMSCs. This function is dependent upon CDK9 and the WAC adaptor protein, which are required for H2B monoubiquitination. Finally, we show that RNF40 is required for the resolution of the H3K4me3/H3K27me3 bivalent poised state on lineage-specific genes during the transition from an inactive to an active chromatin conformation. Thus, these data indicate that H2Bub1 is required for maintaining multipotency of hMSCs and plays a central role in controlling stem cell differentiation.

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Language(s): eng - English
 Dates: 2012-06-072012-06-08
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2012.05.022
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 46 (5) Sequence Number: - Start / End Page: 705 - 713 Identifier: -