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  The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model.

Hülsmann, B. B., Labokha, A., & Görlich, D. (2012). The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. Cell, 150(4), 738-751. doi:10.1016/j.cell.2012.07.019.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-A6AE-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C948-B
Genre: Journal Article

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1481455_Suppl.pdf (Supplementary material), 21MB
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Hülsmann, B. B.1, Author              
Labokha, A.1, Author              
Görlich, D.1, Author              
Affiliations:
1Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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 Abstract: Nuclear pore complexes (NPCs) maintain a permeability barrier between the nucleus and the cytoplasm through FG-repeat-containing nucleoporins (Nups). We previously proposed a “selective phase model” in which the FG repeats interact with one another to form a sieve-like barrier that can be locally disrupted by the binding of nuclear transport receptors (NTRs), but not by inert macromolecules, allowing selective passage of NTRs and associated cargo. Here, we provide direct evidence for this model in a physiological context. By using NPCs reconstituted from Xenopus laevis egg extracts, we show that Nup98 is essential for maintaining the permeability barrier. Specifically, the multivalent cohesion between FG repeats is required, including cohesive FG repeats close to the anchorage point to the NPC scaffold. Our data exclude alternative models that are based solely on an interaction between the FG repeats and NTRs and indicate that the barrier is formed by a sieve-like FG hydrogel.

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Language(s): eng - English
 Dates: 2012-08-122012-08-17
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2012.07.019
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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 150 (4) Sequence Number: - Start / End Page: 738 - 751 Identifier: -