English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Nicotiana attenuata alpha-DIOXYGENASE1 through its production of 2-hydroxylinolenic acid is required for intact plant defense expression against attack from Manduca sexta larvae

Gaquerel, E., Steppuhn, A., & Baldwin, I. T. (2012). Nicotiana attenuata alpha-DIOXYGENASE1 through its production of 2-hydroxylinolenic acid is required for intact plant defense expression against attack from Manduca sexta larvae. New Phytologist, 196(2), 574-585. doi:10.1111/j.1469-8137.2012.04286.x.

Item is

Files

show Files
hide Files
:
ITB385.pdf (Publisher version), 633KB
 
File Permalink:
-
Name:
ITB385.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ITB385s1.docx (Supplementary material), 4MB
 
File Permalink:
-
Name:
ITB385s1.docx
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
OA

Creators

show
hide
 Creators:
Gaquerel, Emmanuel1, Author              
Steppuhn, Anke, Author
Baldwin, Ian Thomas1, Author              
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              

Content

show
hide
Free keywords: -
 Abstract: Nicotiana attenuata α‐DIOXYGENASE1 (α‐DOX1) is an oxylipin‐forming gene elicited during herbivory by fatty acid amino acid conjugates (FACs) contained in oral secretions of Manduca sexta. To understand the roles of Naα‐DOX1 and its major product, 2‐hydroxylinolenic acid (2‐hydroxylinolenic acid), in N. attenuata's anti‐herbivore defenses, we used a transgenic line specifically silenced in Naα‐DOX1 expression (ir‐α‐dox1) and monitored 2‐HOT production in M. sexta‐damaged tissues and its role in influencing the production of direct defense compounds and resistance to this insect. Attack by M. sexta larvae amplified 2‐HOT formation at the feeding sites; a reaction probably facilitated by Naα‐DOX1's high pH optimum which allows 2‐HOT formation to occur in the more alkaline conditions at the feeding sites or potentially in the insect mouth parts after the leaf tissue is ingested. Manduca sexta larvae performed better on ir‐α‐dox1 plants than on wild‐type (WT) plants as a result of attenuated herbivory‐specific JA and 2‐HOT bursts as well as JA‐inducible well‐established defenses (nicotine, caffeoylputrescine and trypsin proteinase inhibitors). Repeated applications of 2‐HOT to wounds before insect feeding partly amplified JA‐controlled defenses and restored the resistance of ir‐α‐dox1 plants. We conclude that 2‐HOT, produced by attack‐activated α‐DOX1 activity, participates in defense activation during insect feeding.

Details

show
hide
Language(s):
 Dates: 20122012-08-312012-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: ITB385
DOI: 10.1111/j.1469-8137.2012.04286.x
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: New Phytologist
  Other : New Phytol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Academic Press.
Pages: - Volume / Issue: 196 (2) Sequence Number: - Start / End Page: 574 - 585 Identifier: ISSN: 0028-646X
CoNE: https://pure.mpg.de/cone/journals/resource/954925334695