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  Line-tension controlled mechanism for influenza fusion.

Risselada, H. J., Marelli, G., Fuhrmans, M., Smirnova, Y. G., Grubmüller, H., Marrink, S. J., et al. (2012). Line-tension controlled mechanism for influenza fusion. PLoS One, 7(6): e38302. doi:10.1371/journal.pone.0038302.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-A91C-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CAB5-F
Genre: Journal Article

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Risselada, H. J.1, Author              
Marelli, G., Author
Fuhrmans, M., Author
Smirnova, Y. G., Author
Grubmüller, H.1, Author              
Marrink, S. J., Author
Müller , M., Author
Affiliations:
1Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              

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 Abstract: Our molecular simulations reveal that wild-type influenza fusion peptides are able to stabilize a highly fusogenic pre-fusion structure, i.e. a peptide bundle formed by four or more trans-membrane arranged fusion peptides. We rationalize that the lipid rim around such bundle has a non-vanishing rim energy (line-tension), which is essential to (i) stabilize the initial contact point between the fusing bilayers, i.e. the stalk, and (ii) drive its subsequent evolution. Such line-tension controlled fusion event does not proceed along the hypothesized standard stalk-hemifusion pathway. In modeled influenza fusion, single point mutations in the influenza fusion peptide either completely inhibit fusion (mutants G1V and W14A) or, intriguingly, specifically arrest fusion at a hemifusion state (mutant G1S). Our simulations demonstrate that, within a linetension controlled fusion mechanism, these known point mutations either completely inhibit fusion by impairing the peptide’s ability to stabilize the required peptide bundle (G1V and W14A) or stabilize a persistent bundle that leads to a kinetically trapped hemifusion state (G1S). In addition, our results further suggest that the recently discovered leaky fusion mutant G13A, which is known to facilitate a pronounced leakage of the target membrane prior to lipid mixing, reduces the membrane integrity by forming a ‘super’ bundle. Our simulations offer a new interpretation for a number of experimentally observed features of the fusion reaction mediated by the prototypical fusion protein, influenza hemagglutinin, and might bring new insights into mechanisms of other viral fusion reactions.

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Language(s): eng - English
 Dates: 2012-06-28
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0038302
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 7 (6) Sequence Number: e38302 Start / End Page: - Identifier: -