English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Evolution of the eukaryotic dynactin complex, the activator of cytoplasmic dynein.

Hammesfahr, B., & Kollmar, M. (2012). Evolution of the eukaryotic dynactin complex, the activator of cytoplasmic dynein. BMC Evolutionary Biology, 12: 95. doi:10.1186/1471-2148-12-95.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-AADF-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C771-1
Genre: Journal Article

Files

show Files
hide Files
:
1491519.pdf (Publisher version), 2MB
Name:
1491519.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Hammesfahr, B.1, Author              
Kollmar, M.1, Author              
Affiliations:
1Research Group of Systems Biology of Motor Protein, MPI for biophysical chemistry, Max Planck Society, ou_578570              

Content

show
hide
Free keywords: -
 Abstract: Background Dynactin is a large multisubunit protein complex that enhances the processivity of cytoplasmic dynein and acts as an adapter between dynein and the cargo. It is composed of eleven different polypeptides of which eight are unique to this complex, namely dynactin1 (p150Glued), dynactin2 (p50 or dynamitin), dynactin3 (p24), dynactin4 (p62), dynactin5 (p25), dynactin6 (p27), and the actin-related proteins Arp1 and Arp10 (Arp11). Results To reveal the evolution of dynactin across the eukaryotic tree the presence or absence of all dynactin subunits was determined in most of the available eukaryotic genome assemblies. Altogether, 3061 dynactin sequences from 478 organisms have been annotated. Phylogenetic trees of the various subunit sequences were used to reveal sub-family relationships and to reconstruct gene duplication events. Especially in the metazoan lineage, several of the dynactin subunits were duplicated independently in different branches. The largest subunit repertoire is found in vertebrates. Dynactin diversity in vertebrates is further increased by alternative splicing of several subunits. The most prominent example is the dynactin1 gene, which may code for up to 36 different isoforms due to three different transcription start sites and four exons that are spliced as differentially included exons. Conclusions The dynactin complex is a very ancient complex that most likely included all subunits in the last common ancestor of extant eukaryotes. The absence of dynactin in certain species coincides with that of the cytoplasmic dynein heavy chain: Organisms that do not encode cytoplasmic dynein like plants and diplomonads also do not encode the unique dynactin subunits. The conserved core of dynactin consists of dynactin1, dynactin2, dynactin4, dynactin5, Arp1, and the heterodimeric actin capping protein. The evolution of the remaining subunits dynactin3, dynactin6, and Arp10 is characterized by many branch- and species-specific gene loss events.

Details

show
hide
Language(s): eng - English
 Dates: 2012-06-22
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/1471-2148-12-95
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Evolutionary Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : BioMed Central
Pages: - Volume / Issue: 12 Sequence Number: 95 Start / End Page: - Identifier: ISSN: 1471-2148
CoNE: /journals/resource/111000136905006