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  Quantitative mass spectrometry-based proteomics: An overview.

Nikolov, M., Schmidt, C., & Urlaub, H. (2012). Quantitative mass spectrometry-based proteomics: An overview. In K. Marcus (Ed.), Quantitative methods in proteomics (pp. 85-100). New York: Humana Pr.; Springer. doi:10.1007/978-1-61779-885-6_7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-C327-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1A74-A
Genre: Book Chapter

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1495645.pdf (Publisher version), 293KB
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 Creators:
Nikolov, M.1, Author              
Schmidt, C.1, Author              
Urlaub, H.1, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Mass spectrometry - Proteomics - Absolute quantification - Relative quantification - Label-free - Stable heavy isotope - Isotope label
 Abstract: In recent years, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review the strategies and problems of the methods currently in use for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.

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Language(s): eng - English
 Dates: 2012-06-302012
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-1-61779-885-6_7
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Title: Quantitative methods in proteomics
Source Genre: Book
 Creator(s):
Marcus, K., Editor
Affiliations:
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Publ. Info: New York : Humana Pr.; Springer
Pages: XV, 539 Volume / Issue: - Sequence Number: - Start / End Page: 85 - 100 Identifier: ISBN: 978-1-61779-884-9

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Title: Methods in molecular biology
Source Genre: Series
 Creator(s):
Walker, J. M., Editor
Affiliations:
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Publ. Info: -
Pages: - Volume / Issue: 893 Sequence Number: - Start / End Page: - Identifier: -