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Abstract:
Protein–RNA complexes play many important roles in diverse cellular functions. They are
involved in a wide variety of different processes in growth and differentiation at the various
stages of the cell cycle. As their function and catalytic activity are directly coupled to the
structural arrangement of their components—proteins and ribonucleic acids—the investigation
of protein–RNA interactions is of great functional and structural importance. Here we discuss
the most prominent examples of protein–RNA complexes and describe some frequently used
purification strategies. We present various techniques and applications of mass spectrometry
to study protein–RNA complexes. We discuss the analysis of intact complexes as well as
proteomics-based and crosslinking-based approaches in which proteins are cleaved into
smaller peptides. This article is part of a Special Section entitled: Understanding genome
regulation and genetic diversity bymass spectrometry
