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  Molecular quantification of Saccharomyces cerevisiae α-pheromone secretion

Rogers, D. W., McConnell, E., & Greig, D. (2012). Molecular quantification of Saccharomyces cerevisiae α-pheromone secretion. FEMS Yeast Research, 12(6), 668-674. doi:10.1111/j.1567-1364.2012.00817.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D365-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-3AC8-B
Genre: Journal Article

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Rogers_et_al-2012-FEMS_Yeast_Research.pdf (Publisher version), 247KB
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Rogers_et_al-2012-FEMS_Yeast_Research.pdf
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 Creators:
Rogers, David W.1, Author              
McConnell, Ellen1, Author              
Greig, Duncan1, Author              
Affiliations:
1Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445640              

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Free keywords: yeast; signal; mating factor; ELISA
 Abstract: Saccharomyces cerevisiae yeast cells court each other by producing an attractive sex pheromone specific to their mating type. Cells detect the sex pheromone from potential mates using a well-defined intracellular signalling cascade that has become a model for studying signal transduction. In contrast, the factors contributing to the production of pheromone itself are poorly characterized, despite the widespread use of the S. cerevisiae a-pheromone secretion pathway in industrial fungal protein expression systems. Progress in understanding pheromone secretion has been hindered by a lack of a precise and quantitative pheromone production assay. Here, we present an ELISA-based method for the quantification of a-pheromone secretion. In the absence of pheromone from the opposite mating type, we found that each cell secretes over 550 mature apheromone peptides per second; 90% of this total was produced from MFa1. The addition of a-pheromone more than doubled total a-pheromone secretion. This technique offers several improvements on current methods for measuring a-pheromone production and will allow detailed investigation of the factors regulating pheromone production in yeast.

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Language(s): eng - English
 Dates: 2012-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 610794
DOI: 10.1111/j.1567-1364.2012.00817.x
Other: 2934/S 39279
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Title: FEMS Yeast Research
  Alternative Title : FEMS Yeast Res
Source Genre: Journal
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Pages: - Volume / Issue: 12 (6) Sequence Number: - Start / End Page: 668 - 674 Identifier: ISSN: 1751-7362 (print)
ISSN: 1751-7370 (online)