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  Diversity of Bacteroidetes in high-altitude saline evaporitic basins in northern Chile

Dorador, C., Meneses, D., Urtuvia, V., Demergasso, C., Vila, I., Witzel, K.-P., et al. (2009). Diversity of Bacteroidetes in high-altitude saline evaporitic basins in northern Chile. Journal of Geophysical Research - Biogeosciences, 114: G00d05. doi:10.1029/2008JG000837.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D574-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D575-1
Genre: Journal Article

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 Creators:
Dorador, Cristina1, Author              
Meneses, Daniela, Author
Urtuvia, Viviana, Author
Demergasso, Cecilia, Author
Vila, Irma, Author
Witzel, Karl-Paul1, Author              
Imhoff, Johannes F., Author
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1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

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 Abstract: The phylum Bacteroidetes represents one of the most abundant bacterial groups of marine and freshwater bacterioplankton. We investigated the diversity of Bacteroidetes in water and sediment samples from three evaporitic basins located in the highlands of northern Chile. We used both 16S rRNA gene clone libraries created with targeted Bacteroidetes-specific primers and separation of specifically amplified gene fragments by denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed a reduced richness of these organisms in samples from Salar de Huasco (two to four DGGE bands) increasing in Salar de Ascotan (two to seven DGGE bands) and Laguna Tebenquiche at Salar de Atacama (four to eight DGGE bands). Cluster analysis (WPGMA) of DGGE bands showed that bands from Salar de Huasco and Salar de Ascotan grouped together and samples from Salar de Atacama formed separate clusters in water and sediment samples, reflecting different Bacteroidetes communities between sites. Most of the sequences analyzed belonged to the family Flavobacteriaceae and clustered with the genera Psychroflexus, Gillisia, Maribacter, Muricauda, Flavobacterium, and Salegentibacter. The most abundant phylotype was highly related to Psychroflexus spp. and was recovered from all three study sites. The similarity of the analyzed sequences with their closest relatives in GenBank was typically <97% and notably lower when compared with type strains, demonstrating the unique character of these sequences. Culture efforts will be necessary to get a better description of the diversity of this group in saline evaporitic basins of northern Chile.

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Language(s): eng - English
 Dates: 2009-08-28
 Publication Status: Published in print
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 Identifiers: eDoc: 439078
DOI: 10.1029/2008JG000837
Other: 2726/S 39046
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Title: Journal of Geophysical Research - Biogeosciences
Source Genre: Journal
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Pages: - Volume / Issue: 114 Sequence Number: G00d05 Start / End Page: - Identifier: ISSN: 0148-0227 (print)