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  Identification and characterization of 14 polymorphic EST-derived microsatellites in eelgrass (Zostera marina)

Oetjen, K., & Reusch, T. B. H. (2007). Identification and characterization of 14 polymorphic EST-derived microsatellites in eelgrass (Zostera marina). Molecular Ecology Notes, 7(5), 777-780. doi:10.1111/j.1471-8286.2007.01694.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D75E-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D75F-5
Genre: Journal Article

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oetjen_2007.pdf (Publisher version), 80KB
 
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 Creators:
Oetjen, Katharina1, Author              
Reusch, Thorsten B. H.1, 2, Author              
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              
2Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              

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Free keywords: conservation; expressed sequence tag; microsatellite; seagrass; SSR; Zostera marina
 Abstract: Zostera marina, the dominant seagrass on the Northern Hemisphere, forms the basis of important but threatened marine ecosystems. Here, we report 14 microsatellite DNA markers derived from an expressed sequence tag library corresponding to a wide range of genes. All loci were moderately to highly polymorphic, with allele numbers ranging from three to eight in a single Wadden Sea population of 48 individuals. Observed heterozygosities ranged from 0.082 to 0.837. Reaction conditions for five pooled polymerase chain reactions are given. The markers will advance the population genetics of seagrasses because they allow indirect tests of selection on closely linked genes.

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Language(s): eng - English
 Dates: 2007-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 321530
DOI: 10.1111/j.1471-8286.2007.01694.x
Other: 2567/S 38720
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Title: Molecular Ecology Notes
Source Genre: Journal
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Pages: - Volume / Issue: 7 (5) Sequence Number: - Start / End Page: 777 - 780 Identifier: ISSN: 1471-8278