English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Lipid extraction has little effect on the δ¹⁵N of aquatic consumers

Ingram, T., Matthews, B., Harrod, C., Stephens, T., Grey, J., Markel, R., et al. (2007). Lipid extraction has little effect on the δ¹⁵N of aquatic consumers. Limnology and Oceanography: Methods, 5, 338-343.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D801-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-D802-B
Genre: Journal Article

Files

show Files
hide Files
:
ingram_2007.pdf (Publisher version), 139KB
 
File Permalink:
-
Name:
ingram_2007.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Ingram, Travis, Author
Matthews, Blake1, Author              
Harrod, Chris1, 2, Author              
Stephens, Tom, Author
Grey, Jonathan1, Author              
Markel, Russell, Author
Mazumder, Asit, Author
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              
2Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

Content

show
hide
Free keywords: -
 Abstract: Proper application of stable isotopes (e. g., delta N-15 and delta C-13) to food web analysis requires an understanding of all nondietary factors that contribute to isotopic variability. Lipid extraction is often used during stable isotope analysis (SIA), because synthesized lipids have a low delta C-13 and can mask the delta C-13 of a consumer's diet. Recent studies indicate that lipid extraction intended to adjust delta C-13 may also cause shifts in delta N-15, but the magnitude of and reasons for the shift are highly uncertain. We examined a large data set (n = 854) for effects of lipid extraction (using Bligh and dyer's [ 1959] chloroform-methanol solvent mixtures) on the delta N-15 of aquatic consumers. We found no effect of chemically extracting lipids on the delta N-15 of whole zooplankton, unionid mussels, and fish liver samples, and found a small increase in fish muscle delta N-15 of similar to 0.4%. We also detected a negative relationship between the shift in delta N-15 following extraction and the C:N ratio in muscle tissue, suggesting that effects of extraction were greater for tissue with lower lipid content. As long as appropriate techniques such as those from Bligh and dyer (1959) are used, effects of lipid extraction on delta N-15 of aquatic consumers need not be a major consideration in the SIA of food webs.

Details

show
hide
Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 334266
Other: 2593/S 38754
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Limnology and Oceanography: Methods
  Alternative Title : Limnol. Oceanogr.: Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 5 Sequence Number: - Start / End Page: 338 - 343 Identifier: ISSN: 1541-5856