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  Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum

Reusch, T. B. H., Rauch, G., & Kalbe, M. (2004). Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum. Molecular Ecology Notes, 4(4), 577-579. doi:10.1111/j.1471-8286.2004.00740.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DA4A-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DA4B-8
Genre: Journal Article

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reusch_2004.pdf (Publisher version), 79KB
 
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 Creators:
Reusch, Thorsten B. H.1, 2, Author              
Rauch, Gisep2, Author              
Kalbe, Martin2, 3, Author              
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              
2Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              
3Research Group Parasitology, Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445643              

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Free keywords: genetic marker; parasite; three-spined stickleback; trematode
 Abstract: We present primers for five polymorphic microsatellite loci in the eye fluke Diplostomum pseudospathaceum (Trematoda), a widely distributed parasite with a complex life cycle used as a model for parasitology and fish immunology. The loci were identified using a GA/CT-enriched genomic library by subtractive hybridization with magnetic particles. All five loci were highly polymorphic, displaying 17 to 61 alleles and heterozygosities ranging from 0.53 to 0.92. We isolated populations of parasites within the first (snail) and second (fish) intermediate host and found small but significant genetic differentiation (FST= 0.012) between the two life stages of the parasite.

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Language(s): eng - English
 Dates: 2004-12
 Publication Status: Published in print
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 Identifiers: eDoc: 205885
DOI: 10.1111/j.1471-8286.2004.00740.x
Other: 2331/S 38208
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Title: Molecular Ecology Notes
Source Genre: Journal
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Pages: - Volume / Issue: 4 (4) Sequence Number: - Start / End Page: 577 - 579 Identifier: ISSN: 1471-8278