English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Evaluation of an innate immune reaction to parasites in earthworms

Field, S. G., Kurtz, J., Cooper, E. L., & Michiels, N. K. (2004). Evaluation of an innate immune reaction to parasites in earthworms. Journal of Invertebrate Pathology, 86(1-2), 45-49. doi:10.1016/j.jip.2004.04.001.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DAF9-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DAFA-F
Genre: Journal Article

Files

show Files
hide Files
:
field_2004.pdf (Publisher version), 841KB
 
File Permalink:
-
Name:
field_2004.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Field, Stuart G., Author
Kurtz, Joachim1, Author              
Cooper, Edwin L., Author
Michiels, Nico K., Author
Affiliations:
1Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              

Content

show
hide
Free keywords: immunocompetence; phenoloxidase; innate immunity; Lumbricus terrestris; coelomocytes; immunoassay; L-3,4-dihydroxyphenylalanine; encapsulation; Monocystis sp.
 Abstract: Encapsulation is an essential process of the invertebrate immune system and includes the prophenoloxidase (proPO) cascade. We present an assay for evaluating this immune response, now newly adapted to earthworms. Coelomic fluid is withdrawn and coelomocytes are stained with L-Dopa. We studied assay repeatability and the correlation between number of PO-active cells and infection level of the parasitic protozoan Monocystis sp. in the earthworm Lumbricus terrestris. Our study showed high assay repeatability although the expected negative relationship between PO-active coelomocytes and parasite load was not observed; yet a suggestion toward a positive relationship was detected. This finding is contrary to previous assumptions that presume coelomocyte concentrations to be the independent variable determining parasite load.

Details

show
hide
Language(s): eng - English
 Dates: 2004-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 176064
DOI: 10.1016/j.jip.2004.04.001
Other: 2298/S 38173
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Invertebrate Pathology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 86 (1-2) Sequence Number: - Start / End Page: 45 - 49 Identifier: ISSN: 0022-2011