English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Impact of violacein-producing bacteria on survival and feeding of bacterivorous nanoflagellates

Matz, C., Deines, P., Boenigk, J., Arndt, H., Eberl, L., Kjelleberg, S., et al. (2004). Impact of violacein-producing bacteria on survival and feeding of bacterivorous nanoflagellates. Applied and Environmental Microbiology, 70(3), 1593-1599. doi:10.1128/AEM.70.3.1593-1599.2004.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DB0E-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DB0F-A
Genre: Journal Article

Files

show Files
hide Files
:
matz_2004.pdf (Publisher version), 849KB
 
File Permalink:
-
Name:
matz_2004.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Matz, Carsten1, Author              
Deines, Peter1, Author              
Boenigk, Jens, Author
Arndt, Hartmut1, Author              
Eberl, Leo, Author
Kjelleberg, Staffan, Author
Jürgens, Klaus1, Author              
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

Content

show
hide
Free keywords: -
 Abstract: We studied the role of bacterial secondary metabolites in the context of grazing protection against protozoans. A model system was used to examine the impact of violacein-producing bacteria on feeding rates, growth, and survival of three common bacterivorous nanoflagellates. Freshwater isolates of Janthinobacterium lividum and Chromobacterium violaceum produced the purple pigment violacein and exhibited acute toxicity to the nanoflagellates tested. High-resolution video microscopy revealed that these bacteria were ingested by the flagellates at high rates. The uptake of less than three bacteria resulted in rapid flagellate cell death after about 20 min and cell lysis within 1 to 2 h. In selectivity experiments with nontoxic Pseudomonas putida MM1, flagellates did not discriminate against pigmented strains. Purified violacein from cell extracts of C violaceum showed high toxicity to nanoflagellates. In addition, antiprotozoal activity was found to positively correlate with the violacein content of the bacterial strains. Pigment synthesis in C violaceum is regulated by an N-acylhomoserine lactone (AHL)-dependent quorum-sensing system. An AHL-deficient, nonpigmented mutant provided high flagellate growth rates, while the addition of the natural C violaceum AHL could restore toxicity. Moreover, it was shown that the presence of violacein-producing bacteria in an otherwise nontoxic bacterial diet considerably inhibited flagellate population growth. Our results suggest that violacein-producing bacteria possess a highly effective survival mechanism which may exemplify the potential of some bacterial secondary metabolites to undermine protozoan grazing pressure and population dynamics.

Details

show
hide
Language(s): eng - English
 Dates: 2004-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 176032
DOI: 10.1128/AEM.70.3.1593-1599.2004
Other: 2302/S 38177
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
  Alternative Title : Appl. Environ. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 70 (3) Sequence Number: - Start / End Page: 1593 - 1599 Identifier: ISSN: 0099-2240