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  Phagocytosis by invertebrate hemocytes: Causes of individual variation in Panorpa vulgaris scorpionflies

Kurtz, J. (2002). Phagocytosis by invertebrate hemocytes: Causes of individual variation in Panorpa vulgaris scorpionflies. Microscopy Research and Technique, 57(6), 456-468. doi:10.1002/jemt.10099.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DD30-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DD31-4
Genre: Journal Article

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Kurtz, Joachim1, Author              
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1Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              

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Free keywords: innate immunity; immunocompetence; host-parasite coevolution; life history; aging
 Abstract: An in vitro phagocytosis assay, adjusted to as little as 1 muL of insect hemolymph, enables the microscopic determination of phagocytosis for single individuals of small insects. Even repeated determination over the lifetime of individuals is possible. This method makes it feasible to study individual variation in invertebrate phagocytic capacity. Possible sources of such variation are reviewed in this article: genetic differences, development, aging, reproduction, presence of parasites, and diverse environmental influences are natural sources of individual variation in phagocytosis. However, the methods used for phagocytosis and microscopic evaluation are also (unwelcome) sources of variation. To optimize incubation time for in vitro phagocytosis, time courses were determined. Furthermore, the reliability of visual counting and image analysis for the microscopic quantification of phagocytosis are compared. The influences of larval development and adult aging on phagocytosis by Panorpa vulgaris hemocytes are subsequently demonstrated. During development, a decrease in hemocyte numbers but a simultaneous increase in the proportion of phagocytosing hemocytes was observed when larvae reached pupation. On the other hand, adults showed a dramatic decrease in phagocytic capacity with age, while cell numbers remained fairly constant. The results show that individual variation in phagocytosis can be determined accurately in small invertebrates and related to its causes. This might be especially interesting in the context of studies relating individual immunocompetence to ecology, life history variation, and behavior. (C) 2002 Wiley-Liss, Inc.

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Language(s): eng - English
 Dates: 2002-06-15
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 3952
ISI: 000176534900005
DOI: 10.1002/jemt.10099
Other: 2106/S 37818
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Title: Microscopy Research and Technique
  Alternative Title : Microsc. Res. Tech.
Source Genre: Journal
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Pages: - Volume / Issue: 57 (6) Sequence Number: - Start / End Page: 456 - 468 Identifier: ISSN: 1059-910X