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  Fluorescent vital labeling to track cestodes in a copepod intermediate host

Kurtz, J., van der Veen, I. T., & Christen, M. (2002). Fluorescent vital labeling to track cestodes in a copepod intermediate host. Experimental Parasitology, 100(1), 36-43. doi:10.1006/expr.2001.4681.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DD8E-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-DD8F-5
Genre: Journal Article

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 Creators:
Kurtz, Joachim1, Author              
van der Veen, Ineke T.1, Author              
Christen, Mira1, Author              
Affiliations:
1Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              

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Free keywords: cestode; copepod; Schistocephalus solidus; Macrocyclops albidus; host-parasite coevolution; fluorescent tracer dyes; parasite tracking; parasite life cycles; immune system; infection
 Abstract: In experimental studies of host-parasite interactions, it is often important to track parasites in their hosts and to discriminate between individual parasites. We used the fluorescent tracer dyes 7-amino-4-chlorornethylcoumarin (CMAC) and, 5-chloromethylfluorescein diacetate (CMFDA) for vital labeling of Schistocephalus solidus (Cestoda) coracidia larvae. Labeling was fast and easy to perform and enabled microscopic detection of parasites appearing as procercoids in the hemocoel of the copepod intermediate host at 3 h after exposure. The label was still visible after 14 days. Extensive controls showed that CMAC (20 muM) labeling did not harm tapeworms or copepods. CMFDA (2 muM) reduced host survival, but the dye concentration can be decreased to avoid this in future studies. The new labeling method presented here has been very useful to track S. solidus parasites. It can be valuable for other parasites also and may be particularly suitable for visualization of individual live macroparasites in invertebrate hosts, for which we are not aware of any other appropriate method. (C) 2002 Elsevier Science (USA).

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Language(s): eng - English
 Dates: 2002-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 3960
ISI: 000175221000006
DOI: 10.1006/expr.2001.4681
Other: 2108/S 37820
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Title: Experimental Parasitology
  Alternative Title : Exp. Parasitol.
Source Genre: Journal
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Pages: - Volume / Issue: 100 (1) Sequence Number: - Start / End Page: 36 - 43 Identifier: ISSN: 0014-4894