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  Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis

Chen, X. S., Rozhdestvensky, T. S., Collins, L. J., Schmitz, J., & Penny, D. (2007). Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis. Nucleic Acids Research, 35, 4619-4628. doi:10.1093/nar/gkm474.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-E5CA-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-E5CB-F
Genre: Journal Article

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NAR_ncRNA_Giardia_2007.pdf (Publisher version), 327KB
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2007
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 Creators:
Chen, Xiaowei Sylvia1, Author              
Rozhdestvensky, Timofey S.2, Author
Collins, Lesley J.1, Author
Schmitz, Jurgen2, Author
Penny, David1, Author
Affiliations:
1Allan Wilson Centre, IMBS, Massey University, Palmerston North, New Zealand and , ou_persistent22              
2Institute of Experimental Pathology (ZMBE), University of Münster, Münster, Germany , ou_persistent22              

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 Abstract: Non-protein-coding RNAs represent a large proportion of transcribed sequences in eukaryotes. These RNAs often function in large RNA–protein complexes, which are catalysts in various RNA-processing pathways. As RNA processing has become an increasingly important area of research, numerous non-messenger RNAs have been uncovered in all the model eukaryotic organisms. However, knowledge on RNA processing in deep-branching eukaryotes is still limited. This study focuses on the identification of non-protein-coding RNAs from the diplomonad parasite Giardia intestinalis, showing that a combined experimental and computational search strategy is a fast method of screening reduced or compact genomes. The analysis of our Giardia cDNA library has uncovered 31 novel candidates, including C/D-box and H/ACA box snoRNAs, as well as an unusual transcript of RNase P, and double-stranded RNAs. Subsequent computational analysis has revealed additional putative C/D-box snoRNAs. Our results will lead towards a future understanding of RNA metabolism in the deep-branching eukaryote Giardia, as more ncRNAs are characterized.

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Language(s): eng - English
 Dates: 2007-02-082007-05-292007
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/nar/gkm474
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Title: Nucleic Acids Research
  Other : Nucleic Acids Res.
Source Genre: Journal
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Publ. Info: Oxford Journals
Pages: - Volume / Issue: 35 Sequence Number: - Start / End Page: 4619 - 4628 Identifier: ISSN: 0301-5610
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000262810