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  Validating serum S100B and neuron-specific enolase as biomarkers for the human brain: A combined serum, gene expression and MRI study

Streitbürger, D. P., Arelin, K., Kratzsch, J., Thiery, J., Steiner, J., Villringer, A., et al. (2012). Validating serum S100B and neuron-specific enolase as biomarkers for the human brain: A combined serum, gene expression and MRI study. PLoS One, 7(8): e43284. doi:10.1371/journal.pone.0043284.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-EA69-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-5C41-C
Genre: Journal Article

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Streitbürger, Daniel Paolo1, Author              
Arelin, Katrin2, 3, 4, Author              
Kratzsch, Jürgen5, Author
Thiery, Joachim3, 5, Author
Steiner, Johann6, 7, Author
Villringer, Arno2, 3, 4, Author              
Mueller, Karsten1, Author              
Schroeter, Matthias L.2, 3, 4, 8, Author              
Affiliations:
1Methods and Development Unit Nuclear Magnetic Resonance, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634558              
2Clinic for Cognitive Neurology, University of Leipzig, Germany, ou_persistent22              
3Leipzig Research Center for Civilization Diseases, University of Leipzig, Germany, ou_persistent22              
4Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
5Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics (ILM), University of Leipzig, Germany, ou_persistent22              
6Department of Psychiatry, Otto von Guericke University Magdeburg, Germany, ou_persistent22              
7Pembroke College, University of Cambridge, United Kingdom, ou_persistent22              
8Consortium for Frontotemporal Lobar Degeneration, Ulm, Germany, ou_persistent22              

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 Abstract: Introduction Former studies have investigated the potential of serum biomarkers for diseases affecting the human brain. In particular the glial protein S100B, a neuro- and gliotrophin inducing plasticity, seems to be involved in the pathogenesis and treatment of psychiatric diseases such as major depression and schizophrenia. Neuron-specific enolase (NSE) is a specific serum marker for neuronal damage. However, the specificity of these biomarkers for cell type and brain region has not been investigated in vivo until now. Methods We acquired two magnetic resonance imaging parameters sensitive to changes in gray and white matter (T1-weighted/diffusion tensor imaging) and obtained serum S100B and NSE levels of 41 healthy subjects. Additionally, we analyzed whole brain gene expressions of S100B in another male cohort of three subjects using the Allen Brain Atlas. Furthermore, a female post mortal brain was investigated using double immunofluorescence labelling with oligodendrocyte markers. Results We show that S100B is specifically related to white matter structures, namely the corpus callosum, anterior forceps and superior longitudinal fasciculus in female subjects. This effect was observed in fractional anisotropy and radial diffusivity – the latest an indicator of myelin changes. Histological data confirmed a co-localization of S100B with oligodendrocyte markers in the human corpus callosum. S100B was most abundantly expressed in the corpus callosum according to the whole genome Allen Human Brain Atlas. In addition, NSE was related to gray matter structures, namely the amygdala. This effect was detected across sexes. Conclusion Our data demonstrates a very high S100B expression in white matter tracts, in particular in human corpus callosum. Our study is the first in vivo study validating the specificity of the glial marker S100B for the human brain, and supporting the assumption that radial diffusivity represents a myelin marker. Our results open a new perspective for future studies investigating major neuropsychiatric disorders.

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Language(s): eng - English
 Dates: 2012-07-232012-08-14
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: PMC: 3422594
PMID: 22916238
DOI: 10.1371/journal.pone.0043284
Other: Epub 2012
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 7 (8) Sequence Number: e43284 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850