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  Expression of the blood-group-related glycosyltransferase B4galnt2 influences the intestinal microbiota in mice

Staubach, F., Künzel, S., Baines, A. C., Yee, A., McGee, B. M., Bäckhed, F., et al. (2012). Expression of the blood-group-related glycosyltransferase B4galnt2 influences the intestinal microbiota in mice. ISME JOURNAL, 6(7), 1345-1355. doi:10.1038/ismej.2011.204.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-EA75-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-EA76-3
Genre: Journal Article

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 Creators:
Staubach, Fabian1, Author              
Künzel, Sven1, Author              
Baines, Andrea C, Author
Yee, Andrew, Author
McGee, Beth M, Author
Bäckhed, Fredrik, Author
Baines, John F.2, Author              
Johnsen, Jill M, Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
2Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

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Free keywords: B4galnt2; blood group; glycosylation; intestine; microbiota; mouse
 Abstract: Glycans on mucosal surfaces have an important role in host–microbe interactions. The locus encoding the blood-group-related glycosyltransferase b-1,4-N-acetylgalactosaminyltransferase 2 (B4galnt2) is subject to strong selective forces in natural house-mouse populations that contain a common allelic variant that confers loss of B4galnt2 gene expression in the gastrointestinal (GI) tract. We reasoned that altered glycan-dependent intestinal host–microbe interactions may underlie these signatures of selection. To determine whether B4galnt2 influences the intestinal microbial ecology, we profiled the microbiota of wild-type and B4galnt2-deficient siblings throughout the GI tract using 16S rRNA gene pyrosequencing. This revealed both distinct communities at different anatomic sites and significant changes in composition with respect to genotype, indicating a previously unappreciated role of B4galnt2 in host–microbial homeostasis. Among the numerous B4galnt2-dependent differences identified in the abundance of specific bacterial taxa, we unexpectedly detected a difference in the pathogenic genus, Helicobacter, suggesting Helicobacter spp. also interact with B4galnt2 glycans. In contrast to other glycosyltransferases, we found that the host intestinal B4galnt2 expression is not dependent on presence of the microbiota. Given the longterm maintenance of alleles influencing B4galnt2 expression by natural selection and the GI phenotypes presented here, we suggest that variation in B4galnt2 GI expression may alter susceptibility to GI diseases such as infectious gastroenteritis.

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Language(s): eng - English
 Dates: 2011-09-222011-05-162011-11-302012-01-262012-07
 Publication Status: Published in print
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 Identifiers: DOI: 10.1038/ismej.2011.204
Other: 2902/S 39247
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Title: ISME JOURNAL
  Abbreviation : ISME J.
Source Genre: Journal
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Pages: - Volume / Issue: 6 (7) Sequence Number: - Start / End Page: 1345 - 1355 Identifier: ISSN: 1751-7362 (print)
ISSN: 1751-7370 (online)
CoNE: /journals/resource/1000000000280510