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  Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases.

Nawale, G. N., Gore, K. R., Höbartner, C., & Pradeepkumar, P. I. (2012). Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases. Chemical communications, 48(77), 9619-9621. doi:10.1039/c2cc35222b.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-EB93-A Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0029-C453-C
Genre: Journal Article

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 Creators:
Nawale, G. N., Author
Gore, K. R., Author
Höbartner, C.1, Author           
Pradeepkumar, P. I., Author
Affiliations:
1Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society, ou_578605              

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 Abstract: The dual modified nucleotide 4′-C-aminomethyl-2′-O-methylthymidine 5′-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2′-O-methyl modification.

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Language(s): eng - English
 Dates: 2012-08-072012
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1039/c2cc35222b
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Title: Chemical communications
Source Genre: Journal
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Pages: - Volume / Issue: 48 (77) Sequence Number: - Start / End Page: 9619 - 9621 Identifier: -