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  Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.

Wagner, E., Lauterbach, M., Kohl, T., Westphal, V., Williams, S. B., Steinbrecher, J. H., et al. (2012). Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction. Circulation Research, 111(4), 402-414. doi:10.1161/CIRCRESAHA.112.274530.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000F-EBF9-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C759-A
Genre: Journal Article

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 Creators:
Wagner, E., Author
Lauterbach, M.1, Author              
Kohl, T., Author
Westphal, V.1, Author              
Williams, S. B., Author
Steinbrecher, J. H., Author
Streich, J., Author
Korff, B., Author
Tuan, M., Author
Hagen, B., Author
Luther, S., Author
Hasenfuss, G., Author
Parlitz, U., Author
Saleet Jafri, M., Author
Hell, S. W.1, Author              
Lederer, W. J., Author
Lehnart, S. E., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: Ca2+ sparks; excitation-contraction coupling; heart failure; T-tubule; super-resolution imaging; calcium signaling
 Abstract: Rationale: Transverse tubules (TTs) couple electric surface signals to remote intracellular Ca2+ release units (CRUs). Diffraction-limited imaging studies have proposed loss of TT components as disease mechanism in heart failure (HF). Objectives: Objectives were to develop quantitative super-resolution strategies for live-cell imaging of TT membranes in intact cardiomyocytes and to show that TT structures are progressively remodeled during HF development, causing early CRU dysfunction. Methods and Results: Using stimulated emission depletion (STED) microscopy, we characterized individual TTs with nanometric resolution as direct readout of local membrane morphology 4 and 8 weeks after myocardial infarction (4pMI and 8pMI). Both individual and network TT properties were investigated by quantitative image analysis. The mean area of TT cross sections increased progressively from 4pMI to 8pMI. Unexpectedly, intact TT networks showed differential changes. Longitudinal and oblique TTs were significantly increased at 4pMI, whereas transversal components appeared decreased. Expression of TT-associated proteins junctophilin-2 and caveolin-3 was significantly changed, correlating with network component remodeling. Computational modeling of spatial changes in HF through heterogeneous TT reorganization and RyR2 orphaning (5000 of 20 000 CRUs) uncovered a local mechanism of delayed subcellular Ca2+ release and action potential prolongation. Conclusions: This study introduces STED nanoscopy for live mapping of TT membrane structures. During early HF development, the local TT morphology and associated proteins were significantly altered, leading to differential network remodeling and Ca2+ release dyssynchrony. Our data suggest that TT remodeling during HF development involves proliferative membrane changes, early excitation-contraction uncoupling, and network fracturing.

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Language(s): eng - English
 Dates: 2012-06-212012-08-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1161/CIRCRESAHA.112.274530
 Degree: -

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Title: Circulation Research
Source Genre: Journal
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Pages: - Volume / Issue: 111 (4) Sequence Number: - Start / End Page: 402 - 414 Identifier: -