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  Three mutations in Escherichia coli that generate transformable functional flagella

Wang, W., Jiang, Z., Westermann, M., & Ping, L. (2012). Three mutations in Escherichia coli that generate transformable functional flagella. Journal of Bacteriology, 194(21), 5856-5863. doi:10.1128/JB.01102-12.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-0C02-E Version Permalink: https://hdl.handle.net/21.11116/0000-0004-8C36-2
Genre: Journal Article

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BOL547.pdf (Publisher version), 4MB
 
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http://dx.doi.org/10.1128/JB.01102-12 (Publisher version)
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 Creators:
Wang, Wenjing1, Author           
Jiang, Zhengzeng1, Author           
Westermann, Martin, Author
Ping, Liyan1, Author           
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              

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 Abstract: Hydrodynamics predicts that swimming bacteria generate a propulsion force when a helical flagellum rotates because rotating helices necessarily translate at a low Reynolds number. It is generally believed that the flagella of motile bacteria are semirigid helices with a fixed pitch determined by hydrodynamic principles. Here, we report the characterization of three mutations in laboratory strains of Escherichia coli that produce different steady-state flagella without losing cell motility. E. coli flagella rotate counterclockwise during forward swimming, and the normal form of the flagella is a left-handed helix. A single amino acid exchange A45G and a double mutation of A48S and S110A change the resting flagella to right-handed helices. The stationary flagella of the triple mutant were often straight or slightly curved at neutral pH. Deprotonation facilitates the helix formation of it. The helical and curved flagella can be transformed to the normal form by torsion upon rotation and thus propel the cell. These mutations arose in the long-term laboratory cultivation. However, flagella are under strong selection pressure as extracellular appendages, and similar transformable flagella would be common in natural environments.

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 Dates: 2012-08-152012-08-24
 Publication Status: Published online
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 Identifiers: Other: BOL547
DOI: 10.1128/JB.01102-12
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Title: Journal of Bacteriology
  Other : J. Bacteriol.
Source Genre: Journal
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Publ. Info: Washington, DC : American Society for Microbiology (ASM)
Pages: - Volume / Issue: 194 (21) Sequence Number: - Start / End Page: 5856 - 5863 Identifier: ISSN: 0021-9193
CoNE: https://pure.mpg.de/cone/journals/resource/954925410823