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  Residues in the pore region of Drosophila TRPA1 dictate sensitivity to thermal stimuli

Wang, H., Schupp, M., Zurborg, S., & Heppenstall, P. A. (2013). Residues in the pore region of Drosophila TRPA1 dictate sensitivity to thermal stimuli. The Journal of Physiology, 591, 185-201. doi:10.1113/jphysiol.2012.242842.

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Wang, Hong1, Author
Schupp, Melanie1, Author
Zurborg, Sandra1, Author              
Heppenstall, Paul A.1, Author
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1Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy, ou_persistent22              

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 Abstract: The capacity to sense temperature is essential for the survival of all animals. At the molecular level, ion channels belonging to the transient receptor potential (TRP) family of channels function as temperature sensors in animals across several phyla. TRP channels are opened directly by changes in temperature and show pronounced sensitivity at their activation range. To determine how temperature activates these channels, we analysed channels belonging to the TRPA family, which detect heat in insects and cold in mammals. By constructing chimeric proteins consisting of human and Drosophila TRPA1 channels, we mapped regions that regulate thermal activation and identified residues in the pore helix that invert temperature sensitivity of TRPA1. From analysis of individual channels we defined the gating reaction of Drosophila TRPA1 and determined how mutagenesis alters the energy landscape for channel opening. Our results reveal specific molecular requirements for thermal activation of TRPA1 and provide mechanistic insight into this process.

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Language(s): eng - English
 Dates: 2012-09-272012-10-012013-01-01
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1113/jphysiol.2012.242842
PMID: 23027824
PMC: PMC3630780
Other: Epub 2012
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Title: The Journal of Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 591 Sequence Number: - Start / End Page: 185 - 201 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_2