English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Novel red fluorophores with superior performance in STED microscopy.

Wurm, C. A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M. L., Schill, H., et al. (2012). Novel red fluorophores with superior performance in STED microscopy. Optical Nanoscopy, 1: 7. doi:10.1186/2192-2853-1-7.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-192A-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C67B-6
Genre: Journal Article

Files

show Files
hide Files
:
1563873.pdf (Publisher version), 3MB
Name:
1563873.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1563873-Suppl.doc (Supplementary material), 269KB
Name:
1563873-Suppl.doc
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/msword / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Wurm, C. A.1, Author              
Kolmakov, K.1, Author              
Göttfert, F., Author
Ta, H., Author
Bossi, M. L.1, Author              
Schill, H.1, Author              
Berning, S.1, Author              
Jakobs, S.2, Author              
Donnert, G.1, Author              
Belov, V. N.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

Content

show
hide
Free keywords: Microscopy, Confocal, STED, Fluorescence, Rhodamines, Bioconjugation, Super-resolution, Nanoscopy, Stimulated emission depletion
 Abstract: In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells.

Details

show
hide
Language(s): eng - English
 Dates: 2012-09-28
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/2192-2853-1-7
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Optical Nanoscopy
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 7 Volume / Issue: 1 Sequence Number: 7 Start / End Page: - Identifier: -