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  Polyamine homeostasis in wild type and phenolamide deficient Arabidopsis thaliana stamens

Fellenberg, C., Ziegler, J., Handrick, V., & Vogt, T. (2012). Polyamine homeostasis in wild type and phenolamide deficient Arabidopsis thaliana stamens. Frontiers in Plant Science, 3: 180. doi:10.3389/fpls.2012.00180.

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http://dx.doi.org/10.3389/fpls.2012.00180 (Publisher version)
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Fellenberg, Christin, Author
Ziegler, Jörg, Author
Handrick, Vinzenz1, Author              
Vogt, Thomas, Author
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1External Organizations, ou_persistent22              

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 Abstract: Polyamines (PAs) like putrescine, spermidine, and spermine are ubiquitous polycationic molecules that occur in all living cells and have a role in a wide variety of biological processes. High amounts of spermidine conjugated to hydroxycinnamic acids are detected in the tryphine of Arabidopsis thaliana pollen grains. Tapetum localized spermidine hydroxycinnamic acid transferase (SHT) is essential for the biosynthesis of these anther specific tris-conjugated spermidine derivatives. Sht knockout lines show a strong reduction of hydroxycinnamic acid amides (HCAAs). The effect of HCAA-deficient anthers on the level of free PAs was measured by a new sensitive and reproducible method using 9-fluorenylmethyl chloroformate (FMOC) and fluorescence detection by HPLC. PA concentrations can be accurately determined even when very limited amounts of plant material, as in the case of A. thaliana stamens, are available. Analysis of free PAs in wild type stamens compared to sht deficient mutants and transcript levels of key PA biosynthetic genes revealed a highly controlled regulation of PA homeostasis in A. thaliana anthers.

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 Dates: 2012-07-232012-08-172012
 Publication Status: Published in print
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 Identifiers: Other: EXT084
DOI: 10.3389/fpls.2012.00180
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Title: Frontiers in Plant Science
  Abbreviation : Front. Plant Sci.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 3 Sequence Number: 180 Start / End Page: - Identifier: ISSN: 1664-462X
CoNE: https://pure.mpg.de/cone/journals/resource/1664462X