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  Chicken Nkx-2.8: A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm.

Reecy, J. M., Cummings, K., Yamada, M., Sosic, D., Chen, C. Y., Eichele, G., et al. (1997). Chicken Nkx-2.8: A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm. Developmental Biology, 188(2), 295-311. doi:10.1006/dbio.1997.8641.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-5012-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C4C8-4
Genre: Journal Article

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 Creators:
Reecy, J. M., Author
Cummings, K., Author
Yamada, M., Author
Sosic, D., Author
Chen, C. Y., Author
Eichele, G.1, Author              
Olson, E. N., Author
Schwartz, R. J., Author
Affiliations:
1Department of Molecular Embryology, Max Planck Institute for Experimental Endocrinology, Max Planck Society, ou_1565140              

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 Abstract: Members of the MK family of homeobox transcription factors regulate critical steps of organogenesis during vertebrate development. In the studies described in this report, we have isolated and functionally characterized the chicken Nkx-2.8 (cNkx-2.8) cDNA and protein and defined the temporal and spatial pattern of cNkx-2.8 gene expression during chicken development. cNkx-2.8 transcripts are first detectable at HH stage 7 in the splanchnopleura. At stage 10(+), the cNkx-2.8 gene is expressed in the linear heart tube and the dorsal half of the vitelline vein. However, after looping, HH stage 13, cNkx-2.8 is no longer expressed in the looped heart tube, but is expressed in the ventral pharyngeal endoderm. At stage 15, in addition to the pharyngeal expression pattern, cNkx-2.8 is expressed in the ectoderm of the pharyngeal arches and the aortic sac. By HH Stage 17, cNkx-2.8 expression is detectable in lateral endoderm of the second and third pharyngeal pouches, the posterior portion of the aortic sac, and the sinus venosus. cNkx-2.8 binds to previously characterized Nkx2-1 and Nkx2-5 DNA-binding sites and overexpression of cNkx-2.8 transactivates a minimal promoter which contains multimerized Nkx-2 DNA-binding sites. In addition, cNkx-2.8 and serum response factor can coactivate a minimal cardiac alpha-actin promoter. These data are consistent with a model in which cNkx-2.8 performs a unique temporally and spatially restricted function in the developing embryonic heart and pharyngeal region. Moreover, the coexpression of cNkx-2.5 and -2.8 raises the possibility that cNkx-2.8 may have a redundant role with cNkx-2.5 in the coalescing heart tube and may play an important role in the transcriptional program(s) that underlies thymus formation. The existence of multiple NK-2 family members and their partially overlapping patterns of expression are discussed Within the framework of a ''Nkx code.''

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Language(s): eng - English
 Dates: 1997-08-15
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1006/dbio.1997.8641
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Title: Developmental Biology
Source Genre: Journal
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Pages: - Volume / Issue: 188 (2) Sequence Number: - Start / End Page: 295 - 311 Identifier: -