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  A high-throughput screen for single gene activities: isolation of apoptosis inducers

Albayrak, T., & Grimm, S. (2003). A high-throughput screen for single gene activities: isolation of apoptosis inducers. Biochemical and Biophysical Research Communications, 304(4), 772-776.

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Genre: Journal Article
Alternative Title : Biochem. Biophys. Res. Commun.

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 Creators:
Albayrak, T.1, Author              
Grimm, S.1, Author              
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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Free keywords: expression cloning; cell death; apoptosis phenotype; 293T cells; respiratory chain; complex II
 Abstract: We describe a novel genetic screen that is performed by transfecting every individual clone of an expression library into a separate population of cells in a high-throughput mode. The screen allows one to achieve a hitherto unattained sensitivity in expression cloning which was exploited in a first read-out to clone apoptosis-inducing genes. This led to the isolation of several genes whose proteins induce distinct phenotypes of apoptosis in 293T cells. One of the isolated genes is the tumor suppressor cytochrome b(L) (cybL), a component of the respiratory chain complex 11, that diminishes the activity of this complex for apoptosis induction. This gene is more efficient and specific for causing cell death than a drug with the same activity. These results suggest further applications, both of the isolated genes and the screen. (C) 2003 Elsevier Science (USA). All rights reserved.

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Language(s): eng - English
 Dates: 2003-05-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41368
ISI: 000182912000030
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Alternative Title : Biochem. Biophys. Res. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 304 (4) Sequence Number: - Start / End Page: 772 - 776 Identifier: ISSN: 0006-291X