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  Plasma fibronectin promotes thrombus growth and stability in injured arterioles

Ni, H. Y., Yuen, P. S. T., Papalia, J. M., Trevithick, J. E., Sakai, T., Fässler, R., et al. (2003). Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proceedings of the National Academy of Sciences of the United States of America, 100(5), 2415-2419.

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Genre: Journal Article
Alternative Title : Proc. Natl. Acad. Sci. U. S. A.

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 Creators:
Ni, H. Y., Author
Yuen, P. S. T., Author
Papalia, J. M., Author
Trevithick, J. E., Author
Sakai, T.1, Author              
Fässler, R.1, Author              
Hynes, R. O., Author
Wagner, D. D., Author
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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 Abstract: Mice lacking both of the best-known platelet ligands, von Willebrand factor and fibrinogen, can still form occlusive thrombi in injured arterioles. The platelets of these animals accumulate excessive amounts of fibronectin (FN). These observations led us to examine the contribution of plasma FN (pFN) to thrombus formation. Inactivation of the FN gene in FN conditional knockout mice reduced pFN levels to <2% and platelet FN to approximate to20% of the levels in similarly treated control mice. The mice were then observed in a model of arterial injury to evaluate their capacity to form thrombi. The deficiency of pFN did not affect the initial platelet adhesion, but a delay of several minutes in thrombus formation was observed in the arterioles of pFN-deficient mice as compared with control mice. The thrombi that formed in the absence of pFN were stably anchored to the vessel wall but continuously shed platelets or small platelet clumps, thus slowing their growth significantly; the platelet/platelet cohesion was apparently diminished. Consequently the occlusion of pFN- deficient vessels was delayed, with the majority of vessels remaining patent at the end of the 40-min observation period. We conclude that, in addition to von Willebrand factor and fibrinogen, FN plays a significant role in thrombus initiation, growth, and stability at arterial shear rates and that deficiency in each of the three platelet ligands has its own specific impact on platelet plug formation.

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Language(s): eng - English
 Dates: 2003-03-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41771
ISI: 000181365000047
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Alternative Title : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 100 (5) Sequence Number: - Start / End Page: 2415 - 2419 Identifier: ISSN: 0027-8424