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  None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion

He, Z. Y., Brakebusch, C., Fässler, R., Kreidberg, J. A., Primakoff, P., & Myles, D. G. (2003). None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion. Developmental Biology, 254(2), 226-237.

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Genre: Journal Article
Alternative Title : Dev. Biol.

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 Creators:
He, Z. Y., Author
Brakebusch, C.1, Author              
Fässler, R.2, Author              
Kreidberg, J. A., Author
Primakoff, P., Author
Myles, D. G., Author
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: integrin; sperm-egg binding; membrane fusion; null mutation; ADAMs
 Abstract: Antibody inhibition and alpha6beta1 ligand binding experiments indicate that the egg integrin alpha6beta1 functions as a receptor for sperm during gamete fusion; yet, eggs null for the alpha6 integrin exhibit normal fertilization. Alternative integrins may be involved in sperm-egg binding and fusion and could compensate for the absence of alpha6beta1. Various beta1 integrins and alphav integrins are present on mouse eggs. Some of these integrins are also reported to be receptors for ADAMs, which are expressed on sperm. Using alpha3 integrin null eggs, we found that the alpha3beta1 integrin was not essential for sperm-egg binding and fusion. Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking all beta1 integrins. We found that the beta1 integrin null eggs were fully functional in fertilization both in vivo and in vitro. Furthermore, neither anti-mouse beta3 integrin function-blocking monoclonal antibody (mAb) nor alphav integrin function-blocking mAb inhibited sperm binding to or fusion with beta1 integrin null eggs. Thus, function of beta3 or alphav integrins does not seem to be involved in compensating for the absence of beta1 integrins. These results indicate that none of the integrins known to be present on mouse eggs or to be ADAM receptors are essential for sperm-egg binding/fusion, and thus, egg integrins may not play the role in gamete fusion previously attributed to them. (C) 2003 Elsevier Science (USA). All rights reserved.

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Language(s): eng - English
 Dates: 2003-02-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41460
ISI: 000181223100006
 Degree: -

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Title: Developmental Biology
  Alternative Title : Dev. Biol.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 254 (2) Sequence Number: - Start / End Page: 226 - 237 Identifier: ISSN: 0012-1606