English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Essential role of the unusual DNA-binding motif of BAG-1 for inhibition of the glucocorticoid receptor

Schmidt, U., Wochnik, G. M., Rosenhagen, M. C., Young, J. C., Hartl, F. U., Holsboer, F., et al. (2003). Essential role of the unusual DNA-binding motif of BAG-1 for inhibition of the glucocorticoid receptor. Journal of Biological Chemistry, 278(7), 4926-4931.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : J. Biol. Chem.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schmidt, U., Author
Wochnik, G. M., Author
Rosenhagen, M. C., Author
Young, J. C.1, Author              
Hartl, F. U.1, Author              
Holsboer, F., Author
Rein, T., Author
Affiliations:
1Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              

Content

show
hide
Free keywords: -
 Abstract: The co-chaperone BAG-1 is involved in the regulation of steroid hormone receptors, including the glucocorticoid receptor (GR). More recently, BAG-1 was found in the nucleus where it decreases GR transactivation. Moreover, nonspecific DNA binding of BAG-1 has been reported. We discovered that of the N- terminal part of BAG-1M, the first 8 amino acids are sufficient for DNA binding, containing a stretch of three lysines and a stretch of three arginines. Changing the spacing between these stretches had no effect on DNA binding. Surprisingly, this small, nonsequence-specific DNA binding domain was nonetheless necessary for the inhibitory function of BAG-1 for GR-dependent transcription, whereas the following serine- and threonine-rich E2X4 repeat domain was not. Mutational analysis of these two domains revealed that only mutants retaining DNA binding capability were able to down-regulate GR-mediated transactivation. Intriguingly, lack of DNA binding could not be functionally rescued by BAG-1M harboring a point mutation abolishing interaction with hsp70. Thus, DNA binding and hsp70 interaction are required in cis. We propose that the nonsequence-specific DNA-binding protein BAG-1 acts at specific chromosomal loci by interacting with other proteins.

Details

show
hide
Language(s): eng - English
 Dates: 2003-02-14
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 39137
ISI: 000180968900076
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Biological Chemistry
  Alternative Title : J. Biol. Chem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 278 (7) Sequence Number: - Start / End Page: 4926 - 4931 Identifier: ISSN: 0021-9258