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  Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography

Medalia, O., Weber, I., Frangakis, A. S., Nicastro, D., Gerisch, G., & Baumeister, W. (2002). Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science, 298(5596), 1209-1213.

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Genre: Journal Article
Alternative Title : Science

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 Creators:
Medalia, O.1, Author              
Weber, I.2, Author              
Frangakis, A. S.1, Author              
Nicastro, D.1, Author              
Gerisch, G.2, Author              
Baumeister, W.1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

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 Abstract: Electron tomography of vitrifed cells is a noninvasive three- dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual micro laments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.

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Language(s): eng - English
 Dates: 2002-11-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41550
ISI: 000179080400042
 Degree: -

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Title: Science
  Alternative Title : Science
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 298 (5596) Sequence Number: - Start / End Page: 1209 - 1213 Identifier: ISSN: 0036-8075